Induction of Carbendazim Resistance in Aspergillus niger through Mutagens

Authors

  • Purnima Sable Department of Botany, S.M.B.S.T. College, Sangamner, Maharashtra-422605, India.
  • L. V. Gangawane Soil microbiology and Pesticides Laboratory, Department of Botany, Dr. Babasaheb Ambedkar Marathwada University, Aurangabad-431004, Maharashtra, India.

Keywords:

Carbendazim, Mutant, Aspergillus niger, Pathogen, Resistant, UV, Ethyl methanesulfonate

Abstract

The sample of Ziziphus fruits infected with different pathogens was collected from the market at different places in Maharashtra. Aspergillus niger was found to be dominant all maximum fruits. Development of Carbendazim resistance in Aspergillus niger due to spontaneous mutation. Ultraviolet rays and chemical mutagens such as like bromyl uracil (BU), ethyl methanesulfonate (EMS) and UV rays, same isolates were highly resistance, some were moderately resistance while few were resistance, highly resistance mutant were more pathogenic. Most sensitive isolates of Aspergillus niger A-9 was selected for this study. The spores of this isolate were treated with UV rays, bromyl uracil & ethyl methanesulfonate, it was seen that EMS treatment gave higher mutants followed by UV, Bu and spontaneous treatment in decreasing manner.

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References

Davidse, L.C. (1981). Resistance to acylalanine fungicides in Phytophthora megasperma f.sp. medicaginis. Neth. J. Plant Pathol., 87(1): 11–24. https://doi.org/10.1007/BF01981396.

Davidse, L.C. & Flach, W. (1977). Differential binding of methyl benzimidazol-2-yl carbamate to fungal tubulin as a mechanism of resistance to this antimitotic agent in mutant strains of Aspergillus nidulans. J. Cell Biol., 72(1): 174–193. https://doi.org/10.1083/jcb.72.1.174.

Gangawane, L.V. & Saler, R.S. (1988). Some physiological aspects of Aspegillus flavus isolates resistant and sensitive to fungicides. Ind. Bot. Reptr., 7(1-2): 61-64.

Gangawane, L.V. & Shaikh, S.A. (1988a). Development of resistance Pythium aphanidermatum to aluminium ethyl phosphate. Indian Phytopath., 41: 638-640.

Horsten, J.A.H.M. (1979). Acquired resistance to systemic fungicides of Septoria nodorum and Cercosporella herpotrichoides in cereals. Dissertation Agricultural University Wageningen, Netherlands, pp. 107.

Reddy, S.M., Rama Pandu, S. & Appa Rao, A. (1979, 1980, 1981). Effect of using combinations and alternate use of fungicides on the in vitro development of fungicide resistance in fungi. Indian Phytopath., 32: 507-517.

Ushiyama, K. (1979). Occurrence of resistant-strains of Penicillium italicum and P. digitatum to thiophanate-methy and benomyl. Bulletin of the Kanagawa Horticultural Experiment Station, 26: 1–6 (in Japanese with English summary).

van Tuyl, J.M. (1977). Genetic aspects of resistance to imazalil in Aspergillus nidulans. Neth. J. Plant Pathol., 83(1): 169–176. https://doi.org/10.1007/BF03041432.

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Published

2012-01-01

How to Cite

Sable, P., & Gangawane, L. V. (2012). Induction of Carbendazim Resistance in Aspergillus niger through Mutagens. Advances in BioScience, 3(1), 35–38. Retrieved from https://journals.sospublication.co.in/ab/article/view/61

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