Molecular Characterization of Nepali Potato Cultivars using Randomly Amplified Polymorphic DNA (RAPD) Markers

Authors

  • Dhurva P. Gauchan Department of Biotechnology, Kathmandu University, Dhulikhel-45200, Kavre, Nepal.
  • Chris Brinegar Division of Natural Sciences, University of Maine, Farmington, Maine-04938, USA.
  • Arjan Hada Department of Biotechnology, Kathmandu University, Dhulikhel-45200, Kavre, Nepal.
  • Dipali Singh Department of Biotechnology, Kathmandu University, Dhulikhel-45200, Kavre, Nepal.

Keywords:

RAPD, Genetic Diversity, Potato, Solanum tuberosum, Genetic Similarity

Abstract

Randomly amplified polymorphic DNA (RAPD) was used to study the genetic diversity of four local cultivars of potato. Amplification with ten arbitrary decamer primers produced 29 different marker bands of which 69.0% were polymorphic. The size range of the amplified DNAs ranged between 370 bp and 2500 bp. On average, 17.5 alleles per genotype were amplified using the RAPD primers. With the selected primers sufficient polymorphism could be detected to allow identification of individual genotypes. A dendrogram displaying the relative genetic similarities between the genotypes showed a range of 55.2-69.0% similarity.

Downloads

Download data is not yet available.

References

del Rio, A.H., Bamberg, J.B., Huaman, Z., Salas, A. & Vega, S.E. (2001). Association of Ecogeographical Variables and RAPD Marker Variation in Wild Potato Populations of the USA. Crop Sci., 41(3): 870–878. https://doi.org/10.2135/cropsci2001.413870x.

Hormaza, J.I., Dollo, L. & Polito, V.S. (1994). Determination of relatedness and geographical movements of Pistacia vera (Pistachio; Anacardiaceae) germplasm by RAPD analysis. Econ. Bot., 48(4): 349–358. https://doi.org/10.1007/BF02862231.

Khatri, B.B. & Shrestha, S.L. (2004). Some Local Potato Cultivars and their Reaction to Viruses. Nepal Agric. Res. J., 5: 19-21.

Kimura, M. & Crow, J.F. (1964). The number of Alleles that can be maintained in a finite population. Genetics, 49(4): 725–738.

Lewontin, R.C. (1972). The Apportionment of Human Diversity. In: Dobzhansky, T., Hecht, M.K., Steere, W.C. (eds.). Evolutionary Biology. Springer, New York. pp 381-398. https://doi.org/10.1007/978-1-4684-9063-3_14.

Moisan-Thiéry, M., Le Hingrat, Y. & Kerlan, M.C. (2001). Potato cultivars identification using molecular markers. Acta Hortic., 546: 471-477. https://doi.org/10.17660/ActaHortic.2001.546.64.

Nei, M. (1973). Analysis of Gene Diversity in Subdivided Populations. Proc. Natl. Acad. Sci. U.S.A, 70(12): 3321–3323. https://doi.org/10.1073/pnas.70.12.3321.

Nei, M. (1978). Estimation of average heterozygosity and genetic distance from a small number of individuals. Genetics, 89(3): 583–590.

Raghunathachari, P., Khanna, V., Singh, U. & Singh, N. (2000). RAPD analysis of genetic variability in Indian scented rice germplasm (Oryza sativa L.). Curr. Sci., 79(7): 994-998.

Rocha, B.H.G., Augustin, E., Pereira, A. Da S., Fortes, G.R. De L. & Peters, J.A. (2002). Caracterização de cultivares de batata através de marcadores moleculares. Rev. Cient. Rural, 7: 42-51.

Shrestha, S.K. (1996). Bacterial wilt of potato in Nepal: spread, losses and magnitude of disease. In: Pradhanang, P.M., Elphinstone, J.G. eds. Integrated management of bacterial wilt of potato: Lessons from the hills of Nepal. Proceedings of a national workshop held at Lumle Agricultural Research Centre, Pokhara, Nepal, 4-5 November 1996: 11-18 pp.

Weising, K., Nybom, H., Wolff, K. & Kahl, G. (2005). DNA fingerprinting in plants: Principles, Methods, and Applications. Boca Raton: Taylor & Francis/CRC press.

Wulff, E.G., Torres, S. & Vigil, E.G. (2002). Protocol for DNA extraction from potato tubers. Plant Mol. Biol. Rep., 20(2): 187. https://doi.org/10.1007/BF02799434.

Downloads

Abstract views: 65 / PDF downloads: 20

Published

2012-04-01

How to Cite

Gauchan, D. P., Brinegar, C., Hada, A., & Singh, D. (2012). Molecular Characterization of Nepali Potato Cultivars using Randomly Amplified Polymorphic DNA (RAPD) Markers. Advances in BioScience, 3(2), 72–75. Retrieved from https://journals.sospublication.co.in/ab/article/view/75

Issue

Vol. 3 No. 2 (2012): April

Section

Articles

License

Copyright (c) 2012 The author(s) retains the copyright of this article.

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Most read articles by the same author(s)