Antagonistic Bioactivity of Endophytic Actinomycetes Isolated from Medicinal Plants

Authors

  • M. Gangwar Department of Microbiology, Punjab Agricultural University, Ludhiana-141004, India.
  • S. Dogra Department of Microbiology, Punjab Agricultural University, Ludhiana-141004, India.
  • N. Sharma Department of Plant Breeding and Genetics, Punjab Agricultural University, Ludhiana-141004, India.

Keywords:

Endophytic actinomycetes, Phytopathogenic fungi, Biocontrol agents, Antagonistic activity

Abstract

Endophytic actinomycetes are promising biocontrol agents for use in agriculture and have been isolated from various plant species. In the present study, 40 endophytic actinomycetes were isolated from roots, stems and leaves of three medicinal plants viz. Aloe vera, Mentha arvensis and Ocimum sanctum. The identification revealed that the majority of the isolates were Streptomyces spp. and the rest were identified as Saccharopolyspora spp., Micromonospora spp. and Actinopolyspora spp. The dual tests revealed that nine endophytic actinomycete isolates displayed a wide spectrum activity against nine fungal phytopathogens. Out of 8 isolates, 90% inhibited the growth of at least one or more phytopathogenic fungi and Saccharopolyspora 0-9 (Out of 8 isolates, 90% inhibited the growth of at least one or more phytopathogenic fungi and Saccharopolyspora 0-9 exhibited antagonistic activity against Aspergillus niger, Aspergillus flavus, Alternaria brassicicola, Botrytis cinerea, Penicillium digitatumFusarium oxysporum, Penicillium pinophilum, Phytophthora dresclea and Colletotrichum falcatum.

Downloads

Download data is not yet available.

References

Aghighi, S., Bonjar, G.H.S., Rawashdeh, R., Batayneh, S. & Saadoun, I. (2004). First Report of Antifungal Spectra of Activity of Iranian Actinomycetes Strains Against Alternaria solani, Alternaria alternate, Fusarium solani, Phytophthora megasperma, Verticillium dahliae and Saccharomyces cerevisiae. Asian J. Plant Sci., 3(4): 463-471. https://dx.doi.org/10.3923/ajps.2004.463.471.

Cao, L., Qiu, Z., You, J., Tan, H. & Zhou, S. (2004). Isolation and characterization of endophytic Streptomyces strains from surface-sterilized tomato (Lycopersicon esculentum) roots. Lett. Appl. Microbiol., 39(5): 425–430. https://doi.org/10.1111/j.1472-765X.2004.01606.x.

Cao, L., Qiu, Z., You, J., Tan, H. & Zhou, S. (2005). Isolation and characterization of endophytic streptomycete antagonists of Fusarium wilt pathogen from surface-sterilized banana roots. FEMS Microbiol. Lett., 247(2): 147–152. https://doi.org/10.1016/j.femsle.2005.05.006.

Coombs, J.T. & Franco, C.M. (2003). Isolation and identification of actinobacteria from surface-sterilized wheat roots. Appl. Environ. Microbiol., 69(9): 5603–5608. https://doi.org/10.1128/AEM.69.9.5603-5608.2003.

Coombs, J.T., Michelsen, P.P. & Franco, C.M.M. (2004). Evaluation of endophytic actinobacteria as antagonists of Gaeumannomyces graminis var. tritici in wheat. Biol. Control, 29(3): 359–366. https://doi.org/10.1016/j.biocontrol.2003.08.001.

Crawford, D.L., Lynch, J.M., Whipps, J.M. & Ousley, M.A. (1993). Isolation and characterization of actinomycete antagonists of a fungal root pathogen. Appl. Environ. Microbiol., 59(11): 3899–3905. https://doi.org/10.1128/aem.59.11.3899-3905.1993.

Araújo, J.M., Silva, A.C. & Azevedo, J.L. (2000). Isolation of endophytic actinomycetes from roots and leaves of maize (Zea mays L.). Braz. Arch. Biol. Technol., 43: 447–451. https://doi.org/10.1590/S1516-89132000000400016.

Khamna, S., Yokota, A. & Lumyong, S. (2009). Actinomycetes isolated from medicinal plant rhizosphere soils: diversity and screening of antifungal compounds, indole-3-acetic acid and siderophore production. World J. Microbiol. Biotechnol., 25(4): 649–655. https://doi.org/10.1007/s11274-008-9933-x.

Sardi, P., Saracchi, M., Quaroni, S., Petrolini, B., Borgonovi, G.E. & Merli, S. (1992). Isolation of endophytic streptomyces strains from surface-sterilized roots. Appl. Environ. Microbiol., 58(8): 2691–2693. https://doi.org/10.1128/aem.58.8.2691-2693.1992.

Taechowisan, T. & Lumyong, S. (2003). Activity of endophytic actinomycetes from roots of Zingiber officinale and Alpinia galanga against phytopathogenic fungi. Ann. Microbiol., 53(3): 291-298.

Taechowisan, T., Peberdy, J.F. & Lumyong, S. (2003). Isolation of endophytic actinomycetes from selected plants and their antifungal activity. World J. Microbiol. Biotechnol., 19(4): 381–385. https://doi.org/10.1023/A:1023901107182.

Tanaka, Y. & Omura, S. (1993). Agroactive compounds of microbial origin. Annu. Rev. Microbiol., 47: 57–87. https://doi.org/10.1146/annurev.mi.47.100193.000421.

Tian, X.L., Cao, L.X., Tan, H.M., Zeng, Q.G., Jia, Y.Y., Han, W.Q. & Zhou, S.N. (2004). Study on the communities of endophytic fungi and endophytic actinomycetes from rice and their antipathogenic activities in vitro. World J. Microbiol. Biotechnol., 20(3): 303–309. https://doi.org/10.1023/B:WIBI.0000023843.83692.3f.

Tokala, R.K., Strap, J.L., Jung, C.M., Crawford, D.L., Salove, M.H., Deobald, L.A., Bailey, J.F. & Morra, M.J. (2002). Novel plant-microbe rhizosphere interaction involving Streptomyces lydicus WYEC108 and the pea plant (Pisum sativum). Appl. Environ. Microbiol., 68(5): 2161–2171. https://doi.org/10.1128/AEM.68.5.2161-2171.2002.

Verma, V.C., Gond, S.K., Kumar, A., Mishra, A., Kharwar, R.N. & Gange, A.C. (2009). Endophytic actinomycetes from Azadirachta indica A. Juss.: Isolation, diversity, and anti-microbial activity. Microb. Ecol., 57(4): 749–756. https://doi.org/10.1007/s00248-008-9450-3.

Yuan, W.M. & Crawford, D.L. (1995). Characterization of streptomyces lydicus WYEC108 as a potential biocontrol agent against fungal root and seed rots. Appl. Environ. Microbiol., 61(8): 3119–3128. https://doi.org/10.1128/aem.61.8.3119-3128.1995.

Downloads

Abstract views: 32 / PDF downloads: 28

Published

2011-10-01

How to Cite

Gangwar, M., Dogra, S., & Sharma, N. (2011). Antagonistic Bioactivity of Endophytic Actinomycetes Isolated from Medicinal Plants. Advances in BioScience, 2(4), 154–157. Retrieved from https://journals.sospublication.co.in/ab/article/view/54

Issue

Section

Articles