Neural Differentiation and Developmental Effects of Honey Bee Venom on PC12 Cells: A Comparison to Nerve Growth Factor (NGF)


  • Mohammad Nabiuni Faculty of Sciences, Department of Biology, University of Tarbiat Moallem, Tehran, Iran.
  • Elham Hoveizi Young Researchers Club, Central Tehran Branch, Islamic Azad University, Tehran, Iran.
  • Kazem Parivar Faculty of Sciences, Department of Biology, University of Tarbiat Moallem, Tehran, Iran.
  • Mahnaz Azarnia Faculty of Sciences, Department of Biology, University of Tarbiat Moallem, Tehran, Iran.
  • Sirus Khodadadi Faculty of Institute of Biochemistry and Biophysics, Tehran University, Tehran, Iran.


Bee venom, PC12 cells, Differentiation, NGF, Neuron


The rat pheochromocytoma cell line, PC12 that in in-vitro condition under inductive factors differentiate and convert into neuron-like cells. Researches have shown that different factors, for example, NGF and bee venom or its components have different effects on proliferation, survival and differentiation of the cells. In this study the PC12 cells were seeded in the culture medium (RPMI-1640) at 5µ103 cell ∕well in poly-D-lysine (0.05mg∕ml) coated 24- well culture plates, allowed to grow for 24hrs, and PC12 cells were treated with NGF for 10 days and were surveyed morphologically in the 1st, 3rd, 5th and 7th days and used various concentrations of honey bee venom for cellular differentiation analysis that the optimum concentrations were 1μg/ml and 3μg/ml, and using NGF with the concentration of 50ng/ml and the venom with the concentrations of 1μg/ml and 3μg/ml simultaneously. The viability of PC12 cells was analyzed by using MTT assay, and cell differentiation were surveyed AChE activity assay and morphologically.


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Nabiuni, M., Hoveizi, E., Parivar, K., Azarnia, M., & Khodadadi, S. (2011). Neural Differentiation and Developmental Effects of Honey Bee Venom on PC12 Cells: A Comparison to Nerve Growth Factor (NGF). Advances in BioScience, 2(3), 129–135. Retrieved from