Evaluation, Antioxidant, Antimitotic and Anticancer Activity of Iron Nanoparticles Prepared by Using Water Extract of Vitis vinifera L. Leaves


  • Rajwa Hasen Essa Department of Biology, College of Science, Al-Mustansiriyah University, Baghdad, Iraq.
  • Mohammad Mahmood Biotechnology Center, Al- Nahrain University, Al-Jadrya, Baghdad-Iraq.
  • Sundus Hameed Ahmed Department of Biology, College of Science, Al-Mustansiriyah University, Baghdad, Iraq.


Fe3O4, Nanoparticles, Allium cepa assay, Anti-inflammatory, Antioxidant


The water extract of Vitis vinifera was used for the rapid synthesis of the iron nanoparticle, which is very simple and eco-friendly in nature. The UV-visible spectroscopy technique was employed to establish the formation of which inhibition onion root growth in a dose-dependent manner. A decrease in the mitotic index may indicate its genotoxic effect on Allium cepa root assay. Nanoparticle was studied for its in vitro anti-inflammatory and antioxidant activity using different models, all the testing, gave significant correlation existed between concentrations of the nanoparticle and percentage inhibition of protein denaturation and scavenging free radicals.  These results clearly indicate that iron nanoparticle is effective against inflammation and free radical-mediated disease.


Download data is not yet available.


Abdulalsalam, H., Ardalan, N.M. & Ahmed, S.H. (2017). Evaluation of anti-inflammatory effect by using iron nanoparticles prepared by Juglans regia water extract. Curr. Res. Microbiol. Biotechnol., 5(4): 1151-1156.

Al-Timimi, S.S. & Hameed, S. (2016). Evaluation of Anti Oxidant and Anti inflammatory Activity of Banana Peels. Advances in Life Science and Technology, 46: 10–15.

Gupta, S.S. (1994). Prospects and perspectives of natural plants products in medicine. Indian J. Pharmacol., 26: 1-12

Havsteen, B. (1983). Flavonoids, a class of natural products of high pharmacological potency. Biochem. Pharmacol., 32(7):1141–1148. https://doi.org/10.1016/0006-2952(83)90262-9.

Lee, S., Son, D., Ryu, J., Lee, Y.S., Jung, S.H., Kang, J., Lee, S.Y., Kim, H.S. & Shin, K.H. (2004). Anti-oxidant activities of Acanthopanax senticosus stems and their lignan components. Arch. Pharm. Res., 27(1): 106–110. https://doi.org/10.1007/BF02980055.

Li, H., Wang, Z. & Liu, Y. (2003). Review in the studies on tannins activity of cancer prevention and anticancer (in Chinese). Zhong yao cai, 26(6): 444–448.

Wadood, A., Ghufran, M., Jamal, S.B., Naeem, M., Khan, A., Ghaffar, R. & Asnad (2013). Phytochemical Analysis of Medicinal Plants Occurring in Local Area of Mardan. Biochem. Anal. Biochem., 2: 144. http://dx.doi.org/10.4172/2161-1009.1000144.

Yadav, R.N.S. & Agarwala, M. (2011). Phytochemical analysis of some medicinal plants. J. Phytol., 3(12): 10-14.

Prema, P. (2011). Chemical Mediated Synthesis of Silver Nanoparticles and its Potential Antibacterial Application. In: Carpi, A. (ed.), Progress in Molecular and Environmental Bioengineering - From Analysis and Modeling to Technology Applications. IntechOpen. http://dx.doi.org/10.5772/22114.

Bala, A., Kar, B., Haldar, P.K., Mazumder, U.K. & Bera, S. (2010). Evaluation of anticancer activity of Cleome gynandra on Ehrlich's Ascites Carcinoma treated mice. J. Ethnopharmacol., 129(1): 131–134. https://doi.org/10.1016/j.jep.2010.03.010.

Jones, P. & Vogt, T. (2001). Glycosyltransferases in secondary plant metabolism: tranquilizers and stimulant controllers. Planta, 213(2): 164–174. https://doi.org/10.1007/s004250000492.

Olajide, O.A., Aderogba, M.A., Adedapo, A.D.A. & Makinde, J.M. (2004). Effects of Anacardium occidentale stem bark extract on in vivo inflammatory models. J. Ethnopharmacol., 95(2): 139–142. https://doi.org/10.1016/j.jep.2004.06.033.

Genuino, H.C., Mazrui, N., Seraji, M.S., Luo, Z. & Hoag, G.E. (2013). Chapter 3 - Green Synthesis of Iron Nanomaterials for Oxidative Catalysis of Organic Environmental Pollutants. In: Suib, S.L. (Ed.), New and Future Developments in Catalysis. Catalysis for Remediation and Environmental. Elsevier. pp. 41-61. https://doi.org/10.1016/B978-0-444-53870-3.00003-4.

Zanella, R. (2012). Metodologías para la síntesis de nanopartículas: controlando forma y tamaño. Mundo Nano. Revista Interdisciplinaria en Nanociencias y Nanotecnología, 5(1): 69-81. https://doi.org/10.22201/ceiich.24485691e.2012.1.45167.

Nadagouda, M.N., Castle, A.B., Murdock, R.C., Hussain, S.M. & Varma, R.S. (2010). In vitro biocompatibility of nanoscale zerovalent iron particles (NZVI) synthesized using tea polyphenols. Green Chem., 12(1): 114–122. https://doi.org/10.1039/B921203P.

Karadeniz, F., Durst, R.W. & Wrolstad, R.E. (2000). Polyphenolic composition of raisins. J. Agric. Food Chem., 48(11): 5343–5350. https://doi.org/10.1021/jf0009753.

Liu, C.P., Tsai, W.J., Lin, Y.L., Liao, J.F., Chen, C.F. & Kuo, Y.C. (2004). The extracts from Nelumbo Nucifera suppress cell cycle progression, cytokine genes expression, and cell proliferation in human peripheral blood mononuclear cells. Life Sci., 75(6): 699–716. https://doi.org/10.1016/j.lfs.2004.01.019.

Freshney, R.I. (2010). Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications. 6th Edition. Wiley-Blackwell, Hoboken, N.J. https://doi.org/10.1002/9780470649367.

Mao, A.A. (2002). Oroxylum indicum Vent. - A potential anticancer medicinal plant. Indian J. Tradit. Knowl., 1(1): 17–21.

Kumar, D.R.N., George, V.C., Suresh, P.K. & Kumar, R.A. (2012). Cytotoxicity, Apoptosis Induction and Anti-Metastatic Potential of Oroxylum indicum in Human Breast Cancer Cells. Asian Pac. J. Cancer Prev., 13(6): 2729–2734. https://doi.org/10.7314/apjcp.2012.13.6.2729.

Singleton, V.L. & Rossi, J.A. (1965). Colorimetry of Total Phenolics with Phosphomolybdic-Phosphotungstic Acid Reagents. Am. J. Enol. Vitic., 16(3): 144–158.

Marinova, D., Ribarova, F. & Atanassova, M. (2005). Total phenolics and flavonoids in Bulgarian fruits and vegetables. J. Univ. Chem. Technol. Metallurgy, 40(3): 255–260.


Abstract views: 38 / PDF downloads: 12



How to Cite

Essa, R. H., Mahmood, M., & Ahmed, S. H. (2017). Evaluation, Antioxidant, Antimitotic and Anticancer Activity of Iron Nanoparticles Prepared by Using Water Extract of Vitis vinifera L. Leaves. Advances in BioScience, 8(3), 67–73. Retrieved from https://journals.sospublication.co.in/ab/article/view/225