Blastocystis hominis: Is it a cause of diarrhea?

Authors

  • Amnah A. Bugis Department of Medical Laboratory Technology, Faculty of Applied Medical Sciences, King Abdulaziz University, P.O. Box 80324, Jeddah 21589, Saudi Arabia.
  • Banan A. Mallah Department of Medical Laboratory Technology, Faculty of Applied Medical Sciences, King Abdulaziz University, P.O. Box 80324, Jeddah 21589, Saudi Arabia.
  • Bayan A. Mallah Department of Medical Laboratory Technology, Faculty of Applied Medical Sciences, King Abdulaziz University, P.O. Box 80324, Jeddah 21589, Saudi Arabia.
  • Haytham A. Zakai Department of Medical Laboratory Technology, Faculty of Applied Medical Sciences, King Abdulaziz University, P.O. Box 80324, Jeddah 21589, Saudi Arabia.

Keywords:

Blastocystis hominis, Diarrhea prevalence, Jeddah

Abstract

Blastocystis hominis can be found in the fecal sample of both healthy individuals without symptoms and those having gastrointestinal problems. Therefore, the pathogenicity and pathophysiology remain controversial. The aim of this study was to determine if Blastocystis hominis is a causative agent of diarrhea and investigates its prevalence among patients visiting King Abdulaziz University Hospital, Jeddah, Saudi Arabia from January 2013 till June 2015 and to determine any significant correlation between age, gender, nationality and other parasitic infection in the presence of Blastocystis hominis. Data were collected from Parasitology lab, using the database at King Abdulaziz University Hospital, Jeddah, Saudi Arabia and statistical analysis were done by utilizing Statistical package for social science program (SPSS). Out of 3,035 of total stool analysis requested, 157 samples were positive for B. hominis (5.2%). 80 (51.6%) were males, and 76 (48.4%) were females. The distribution of B. hominis infection was elevated in 31-50 aged groups (25.5%). There was a statistically significant correlation between the presence of Blastocystis and diarrhea at p < 0.05 and there was no correlation between age, sex, nationality and other parasites with positivity of B. hominis. Blastocystis can be considered as a causative agent of diarrhea. Further studies need to be done in order to investigate its role in pathogenicity and more data must be revised to assess the pathogenicity of Blastocystis subtypes.

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References

Zakai, H.A., Abunar, A.A. & Khoudari, A.A. (2014). Intestinal parasitic infections among the household staff working in Saudi family houses Jeddah, Saudi Arabia. J. Adv. Lab. Res. Biol., 5(2): 18–20.

Hawash, Y.A., Dorgham, L., Amir, E.M. & Sharaf, O.F. (2015). Prevalence of Intestinal Protozoa among Saudi Patients with Chronic Renal Failure: A Case-Control Study. J. Trop. Med., vol. 2015, Article ID 563478. https://doi.org/10.1155/2015/563478.

Rostami Nejad, M., Nazemalhosseini Mojarad, E., Dabiri, H., Nochi, Z., Pourhoseingholi, M.A., Sahebekhtiari, N., Habibi, M. & Zali, M.R. (2010). A case-control study of Blastocystis hominis among Iranian population. East Afr. J. Public Health, 7(1): 103-106. http://dx.doi.org/10.4314/eajph.v7i1.64706.

Rajamanikam, A. & Govind, S.K. (2013). Amoebic forms of Blastocystis spp. - evidence for a pathogenic role. Parasit. Vectors, 6: 295. https://doi.org/10.1186/1756-3305-6-295.

Zaglool, A.M., Khodari, A.W. & Farooq, U. (2012). Blastocystis hominis and allergic skin diseases; a single centre experience. J. Health Sci., 2(1): 66–69. https://doi.org/10.17532/jhsci.2012.66.

Yakoob, J., Jafri, W., Beg, M.A., Abbas, Z., Naz, S., Islam, M. & Khan, R. (2010). Blastocystis hominis and Dientamoeba fragilis in patients fulfilling irritable bowel syndrome criteria. Parasitol. Res., 107(3): 679–684. https://doi.org/10.1007/s00436-010-1918-7.

Hayashi, M., Inamori, M., Goto, K., Akiyama, T., Fujita, K., Ikeda, I., Fujisawa, T., Takahashi, H., Yoneda, M., Hara, K., Abe, Y., Kirikoshi, H., Kubota, K., Saito, S., Ueno, N., Nakajima, A., Hamada, Y., Fukutomi, H. & Satsuta, H. (2006). Blastocystis hominis infection in patient with regular dialysis. J. Gastroenterol., 41(6): 605–606. https://doi.org/10.1007/s00535-006-1817-y.

Stensvold, C.R., Christiansen, D.B., Olsen, K.E. & Nielsen, H.V. (2011). Blastocystis sp. subtype 4 is common in Danish Blastocystis-positive patients presenting with acute diarrhea. Am. J. Trop. Med. Hyg., 84(6): 883–885. https://doi.org/10.4269/ajtmh.2011.11-0005.

Beyhan, Y.E., Yilmaz, H., Cengiz, Z.T. & Ekici, A. (2015). Clinical significance and prevalence of Blastocystis hominis in Van, Turkey. Saudi Med. J., 36(9): 1118–1121. https://doi.org/10.15537/smj.2015.9.12444.

Jones, M.S., 2nd, Ganac, R.D., Hiser, G., Hudson, N.R., Le, A. & Whipps, C.M. (2008). Detection of Blastocystis from stool samples using real-time PCR. Parasitol. Res., 103(3): 551–557. https://doi.org/10.1007/s00436-008-1006-4.

Bart, A., Wentink-Bonnema, E.M.S., Gilis, H., Verhaar, N., Wassenaar, C.J.A., van Vugt, M., Goorhuis, A. & van Gool, T. (2013). Diagnosis and subtype analysis of Blastocystis sp. in 442 patients in a hospital setting in the Netherlands. BMC Infect. Dis., 13: 389. https://doi.org/10.1186/1471-2334-13-389.

Chan, K.H., Chandramathi, S., Suresh, K., Chua, K.H. & Kuppusamy, U.R. (2012). Effects of symptomatic and asymptomatic isolates of Blastocystis hominis on colorectal cancer cell line, HCT116. Parasitol. Res., 110(6): 2475–2480. https://doi.org/10.1007/s00436-011-2788-3.

Dagci, H., Kurt, Ö., Demirel, M., Mandiracioglu, A., Aydemir, S., Saz, U., Bart, A. & VAN Gool, T. (2014). Epidemiological and diagnostic features of blastocystis infection in symptomatic patients in Izmir province, Turkey. Iran. J. Parasitol., 9(4): 519–529.

Leelayoova, S., Taamasri, P., Rangsin, R., Naaglor, T., Thathaisong, U. & Mungthin, M. (2002). In-vitro cultivation: a sensitive method for detecting Blastocystis hominis. Ann. Trop. Med. Parasitol., 96(8): 803–807. https://doi.org/10.1179/000349802125002275.

Whipps, C.M., Boorom, K., Bermudez, L.E. & Kent, M.L. (2010). Molecular characterization of Blastocystis species in Oregon identifies multiple subtypes. Parasitol. Res., 106(4): 827–832. https://doi.org/10.1007/s00436-010-1739-8.

El Safadi, D., Meloni, D., Poirier, P., Osman, M., Cian, A., Gaayeb, L., Wawrzyniak, I., Delbac, F., El Alaoui, H., Delhaes, L., Dei-Cas, E., Mallat, H., Dabboussi, F., Hamze, M. & Viscogliosi, E. (2013). Molecular epidemiology of Blastocystis in Lebanon and correlation between subtype 1 and gastrointestinal symptoms. Am. J. Trop. Med. Hyg., 88(6): 1203–1206. https://doi.org/10.4269/ajtmh.12-0777.

Leder, K., Hellard, M.E., Sinclair, M.I., Fairley, C.K. & Wolfe, R. (2005). No correlation between clinical symptoms and Blastocystis hominis in immunocompetent individuals. J. Gastroenterol. Hepatol., 20(9): 1390–1394. https://doi.org/10.1111/j.1440-1746.2005.03868.x.

O'Gorman, M.A., Orenstein, S.R., Proujansky, R., Wadowsky, R.M., Putnam, P.E. & Kocoshis, S.A. (1993). Prevalence and characteristics of Blastocystis hominis infection in children. Clin. Pediatr., 32(2): 91–96. https://doi.org/10.1177/000992289303200206.

Al-Megrin, W.A. (2010). Intestinal parasites infection among immunocompromised patients in Riyadh, Saudi Arabia. Pak. J. Biol. Sci., 13(8): 390–394. https://doi.org/10.3923/pjbs.2010.390.394.

Al-Binali, A.M., Bello, C.S., El-Shewy, K. & Abdulla, S.E. (2006). The prevalence of parasites in commonly used leafy vegetables in South Western, Saudi Arabia. Saudi Med. J., 27(5): 613–616.

Khan, Z.A. & Alkhalife, I.S. (2005). Prevalence of Blastocystis hominis among "healthy" food handlers in Dammam, Saudi Arabia. J. Egypt. Soc. Parasitol., 35(2): 395–401.

Amin, A.M. (1997). Blastocystis hominis among apparently healthy food handlers in Jeddah, Saudi Arabia. J. Egypt. Soc. Parasitol., 27(3): 817–823.

Duda, A., Kosik-Bogacka, D., Lanocha-Arendarczyk, N., Kołodziejczyk, L. & Lanocha, A. (2015). The prevalence of Blastocystis hominis and other protozoan parasites in soldiers returning from peacekeeping missions. Am. J. Trop. Med. Hyg., 92(4): 805–806. https://doi.org/10.4269/ajtmh.14-0344.

Coyle, C.M., Varughese, J., Weiss, L.M. & Tanowitz, H.B. (2012). Blastocystis: to treat or not to treat. Clin. Infect. Dis., 54(1): 105–110. https://doi.org/10.1093/cid/cir810.

Tai, W.P., Hu, P.J., Wu, J. & Lin, X.C. (2011). Six ulcerative colitis patients with refractory symptoms co-infective with Blastocystis hominis in China. Parasitol. Res., 108(5): 1207–1210. https://doi.org/10.1007/s00436-010-2164-8.

Nourrisson, C., Scanzi, J., Pereira, B., NkoudMongo, C., Wawrzyniak, I., Cian, A., Viscogliosi, E., Livrelli, V., Delbac, F., Dapoigny, M. & Poirier, P. (2014). Blastocystis is associated with decrease of fecal microbiota protective bacteria: comparative analysis between patients with irritable bowel syndrome and control subjects. PLoS One, 9(11): e111868. https://doi.org/10.1371/journal.pone.0111868.

Matiut, D.S. & Hritcu, L. (2015). The pathogenic role of Blastocystis isolated from patients with irritable bowel syndrome and colitis from Iasi, Romania. Acta Parasitol., 60(1): 116–123. https://doi.org/10.1515/ap-2015-0015.

Dunn, L.A., Tan, K.S., Vanelle, P., Juspin, T., Crozet, M.D., Terme, T., Upcroft, P. & Upcroft, J.A. (2012). Development of metronidazole-resistant lines of Blastocystis sp. Parasitol Res., 111(1): 441–450. https://doi.org/10.1007/s00436-012-2860-7.

Poirier, P., Meloni, D., Nourrisson, C., Wawrzyniak, I., Viscogliosi, E., Livrelli, V. & Delbac, F. (2014). Molecular subtyping of Blastocystis spp. using a new rDNA marker from the mitochondria-like organelle genome. Parasitology, 141(5): 670–681. https://doi.org/10.1017/S0031182013001996.

Abu-Madi, M., Aly, M., Behnke, J.M., Clark, C.G. & Balkhy, H. (2015). The distribution of Blastocystis subtypes in isolates from Qatar. Parasites Vectors, 8: 465. https://doi.org/10.1186/s13071-015-1071-3.

Lee, L.I., Chye, T.T., Karmacharya, B.M. & Govind, S.K. (2012). Blastocystis sp.: waterborne zoonotic organism, a possibility? Parasites Vectors, 5: 130. https://doi.org/10.1186/1756-3305-5-130.

BIOSEPAR - Diagnostik für die Zukunft [Online] [2016]. Biosepar GmbH. Retrieved on 23/04/2016 from: http://www.biosepar.de/DownloadsEN.htm.

Our Products – Hemosure, Inc. [Online] [2016]. Hemosure, Inc. Retrieved on 23/04/2016 from: http://hemosure.com/hospital-laboratory/products/.

Koshak, E.A. & Zakai, H.A. (2003). A spectrum of pathogenic and non-pathogenic intestinal parasites in pre-employment medical check-up for workers and their families. J. Fam. Community Med., 10: 47-53.

Ozçakir, O., Güreser, S., Ergüven, S., Yilmaz, Y.A., Topaloğlu, R. & Hasçelik, G. (2007). Characteristics of Blastocystis hominis infection in a Turkish university hospital. Turkiye Parazitol. Derg., 31(4): 277–282.

Stensvold, C.R., Lewis, H.C., Hammerum, A.M., Porsbo, L.J., Nielsen, S.S., Olsen, K.E., Arendrup, M.C., Nielsen, H.V. & Mølbak, K. (2009). Blastocystis: unravelling potential risk factors and clinical significance of a common but neglected parasite. Epidemiol. Infect., 137(11): 1655–1663. https://doi.org/10.1017/S0950268809002672.

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Published

2017-01-01

How to Cite

Bugis, A. A., Mallah, B. A., Mallah, B. A., & Zakai, H. A. (2017). Blastocystis hominis: Is it a cause of diarrhea?. Advances in BioScience, 8(1), 1–5. Retrieved from https://journals.sospublication.co.in/ab/article/view/220

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