CRP in Relation To CVD

Abstract

Cardiovascular diseases are leading to death in both men and women. One of the risk factors mainly involved in CVD and CRP as inflammatory markers. Atherosclerosis is a disease characterized by chronic inflammation and is now widely accepted to be inflammatory diseases. Clinical manifestations include coronary heart disease [CHD], cerebrovascular disease and peripheral vascular disease. In this study focus on CRP, this protein will be expressed on CVD patients and the relationship between Insulin resistance leads to cardiovascular disease and diabetic diseases occurs in young age & adult age peoples were living worldwide. The CVD patient and normal sample were collected respectively in hospital. These samples were estimated by protein estimation and analysis of CRP using SDS PAGE gel electrophoresis and assay method of western blot done by the color development system using primary & secondary antibody and also DAB kit. The measurements of BMI correlate to the CRP and INSULIN level. Increased BMI that can lead to obesity occurs in both men & women. The CRP protein level increases the chance of cardiovascular disease and insulin level also increased, the patient should develop diabetes. These diseases are analyzed by diagnostic methods. In human CRP was synthesized by hepatocytes and secreted into serum and also whether CRP as a human acute phase protein synthesis by PBMC and isolate the lymphocytes in cultured medium and protein was isolated & estimated due to analysis of CRP using SDS PAGE Gel Electrophoresis. From the preliminary results, there is an evidence of raise in CRP among CVD patients which is to be dealt in detail in the near future to use CRP proteins as a marker in CVD detection.