Prevalence of Cryptosporidium and Giardia lamblia in Water Samples from Jeddah and Makkah Cities
Keywords:
Cryptosporidium, Giardia, Water contamination, Jeddah, MakkahAbstract
Water contamination by Giardia lamblia and Cryptosporidium is one of the causes of diarrhoea throughout the world. A total of 161 and 84 samples were collected from Jeddah and Makkah cities, respectively. Each sample was concentrated by double centrifugation and the sediment was examined as a wet smear and after staining with Trichrome and Kinyoun stains. The results showed that 56 (35%) and 1 (0.62 %) samples of Jeddah were positive for the oocyst of Cryptosporidium and cyst of Giardia, whereas only 21 (25%) and 2 (2.4 %) samples of Makkah showed positivity for oocysts and cyst of these parasites. Overall Cryptosporidium contamination in bottled water and water from filling stations was 6.8% and 17.4%, respectively. Maximum contamination for Cryptosporidium was recorded in tap water which was 51% and 25% in Jeddah and Makkah, respectively.
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Ryu, H., Alum, A., Mena, K.D. & Abbaszadegan, M. (2007). Assessment of the risk of infection by Cryptosporidium and Giardia in non-potable reclaimed water. Water Sci. Technol., 55(1-2): 283–290. https://doi.org/10.2166/wst.2007.047.
Robertson, L.J., Forberg, T. & Gjerde, B.K. (2008). Giardia cysts in sewage influent in Bergen, Norway 15-23 months after an extensive waterborne outbreak of giardiasis. J. Appl. Microbiol., 104(4): 1147–1152. https://doi.org/10.1111/j.1365-2672.2007.03630.x.
Tzipori, S. (1983). Cryptosporidiosis in animals and humans. Microbiol. Rev., 47(1): 84–96. https://doi.org/10.1128/mr.47.1.84-96.1983.
Forgacs, P., Tarshis, A., Ma, P., Federman, M., Mele, L., Silverman, M.L. & Shea, J.A. (1983). Intestinal and bronchial cryptosporidiosis in an immunodeficient homosexual man. Ann. Intern. Med., 99(6): 793–794. https://doi.org/10.7326/0003-4819-99-6-793.
Ma, P., Villanueva, T.G., Kaufman, D. & Gillooley, J.F. (1984). Respiratory cryptosporidiosis in the acquired immune deficiency syndrome. Use of modified cold Kinyoun and Hemacolor stains for rapid diagnoses. JAMA, 252(10): 1298–1301. https://doi.org/10.1001/jama.1984.03350100028024.
Fayer, R. & Ungar, B.L. (1986). Cryptosporidium spp. and cryptosporidiosis. Microbiol. Rev., 50(4): 458–483. https://doi.org/10.1128/mr.50.4.458-483.1986.
Hunter, P.R. & Nichols, G. (2002). Epidemiology and clinical features of Cryptosporidium infection in immunocompromised patients. Clin. Microbiol. Rev., 15(1): 145–154. https://doi.org/10.1128/CMR.15.1.145-154.2002.
Raccurt, C.P., Brasseur, P., Verdier, R.I., Li, X., Eyma, E., Stockman, C.P., Agnamey, P., Guyot, K., Totet, A., Liautaud, B., Nevez, G., Dei-Cas, E. & Pape, J.W. (2006). Human cryptosporidiosis and Cryptosporidium spp. in Haiti. Trop. Med. Int. Health., 11(6): 929–934. https://doi.org/10.1111/j.1365-3156.2006.01631.x.
Sanad, M.M. & Al-Malki, J.S. (2007). Cryptosporidiosis among immunocompromised patients in Saudi Arabia. J. Egypt. Soc. Parasitol., 37(2 Suppl): 765–774.
Ajjampur, S.S., Sankaran, P. & Kang, G. (2008). Cryptosporidium species in HIV-infected individuals in India: an overview. Natl. Med. J. India, 21(4): 178–184.
Nahrevanian, H. & Assmar, M. (2008). Cryptosporidiosis in immunocompromised patients in the Islamic Republic of Iran. J. Microbiol. Immunol. Infect., 41(1): 74–77.
Eisenstein, L., Bodager, D. & Ginzl, D. (2008). Outbreak of giardiasis and cryptosporidiosis associated with a neighborhood interactive water fountain--Florida, 2006. J. Environ. Health, 71(3): 18–22.
Nishi, L., Baesso, M.L., Santana, R.G., Fregadolli, P., Falavigna, D.L. & Falavigna-Guilherme, A.L. (2009). Investigation of Cryptosporidium spp. and Giardia spp. in a public water-treatment system. Zoonoses Public Health, 56(5): 221–228. https://doi.org/10.1111/j.1863-2378.2008.01189.x.
Daly, E.R., Roy, S.J., Blaney, D.D., Manning, J.S., Hill, V.R., Xiao, L. & Stull, J.W. (2010). Outbreak of giardiasis associated with a community drinking-water source. Epidemiol. Infect., 138(4): 491–500. https://doi.org/10.1017/S0950268809990744.
Al-Mohammed, H.I. (2011). Genotypes of Giardia intestinalis clinical isolates of gastrointestinal symptomatic and asymptomatic Saudi children. Parasitol. Res., 108(6): 1375–1381. https://doi.org/10.1007/s00436-010-2033-5.
Garcia, L.S. (2001). Modified Kinyoun's Acid-Fast Stain (Cold). Diagnostic Medical Parasitology, 4th edn, ASM Press, Washington, DC, p. 723.
Chalmers, R.M., Campbell, B.M., Crouch, N., Charlett, A. & Davies, A.P. (2011). Comparison of diagnostic sensitivity and specificity of seven Cryptosporidium assays used in the UK. J. Med. Microbiol., 60(Pt 11): 1598–1604. https://doi.org/10.1099/jmm.0.034181-0.
Magi, B., Canocchi, V., Tordini, G., Cellesi, C. & Barberi, A. (2006). Cryptosporidium infection: diagnostic techniques. Parasitol. Res., 98(2): 150–152. https://doi.org/10.1007/s00436-005-0050-6.
Rigo, C.R. & Franco, R.M. (2002). Comparison between the modified Ziehl-Neelsen and Acid-Fast-Trichrome methods for fecal screening of Cryptosporidium parvum and Isospora belli. Rev. Soc. Bras. Med. Trop., 35(3): 209–214. https://doi.org/10.1590/s0037-86822002000300002.
Tee, G.H., Moody, A.H., Cooke, A.H. & Chiodini, P.L. (1993). Comparison of techniques for detecting antigens of Giardia lamblia and Cryptosporidium parvum in faeces. J. Clin. Pathol., 46(6): 555–558. https://doi.org/10.1136/jcp.46.6.555.
Wallis, P.M., Erlandsen, S.L., Isaac-Renton, J.L., Olson, M.E., Robertson, W.J. & van Keulen, H. (1996). Prevalence of Giardia cysts and Cryptosporidium oocysts and characterization of Giardia spp. isolated from drinking water in Canada. Appl. Environ. Microbiol., 62(8): 2789–2797. https://doi.org/10.1128/aem.62.8.2789-2797.1996.
Carmena, D., Aguinagalde, X., Zigorraga, C., Fernández-Crespo, J.C. & Ocio, J.A. (2007). Presence of Giardia cysts and Cryptosporidium oocysts in drinking water supplies in northern Spain. J. Appl. Microbiol., 102(3): 619–629. https://doi.org/10.1111/j.1365-2672.2006.03193.x.
Al-Braiken, F.A., Amin, A., Beeching, N.J., Hommel, M. & Hart, C.A. (2003). Detection of Cryptosporidium amongst diarrhoeic and asymptomatic children in Jeddah, Saudi Arabia. Ann. Trop. Med. Parasitol., 97(5): 505–510. https://doi.org/10.1179/000349803235002470.
Areeshi, M.Y., Beeching, N.J. & Hart, C.A. (2007). Cryptosporidiosis in Saudi Arabia and neighboring countries. Ann. Saudi Med., 27(5): 325–332. https://doi.org/10.5144/0256-4947.2007.325.
Iqbal, J., Khalid, N. & Hira, P.R. (2011). Cryptosporidiosis in Kuwaiti children: association of clinical characteristics with Cryptosporidium species and subtypes. J. Med. Microbiol., 60(Pt 5): 647–652. https://doi.org/10.1099/jmm.0.028001-0.
Kasim, A.A. & Elhelu, M.A. (1983). Giardiasis in Saudi Arabia. Acta Trop., 40(2): 155–158.
Khan, Z.H., Namnyak, S.S., Al Jama, A.A. & Madan, I. (1988). Prevalence of cryptosporidiosis in Dammam and Alkhobar, Saudi Arabia. Ann. Trop. Paediatr., 8(3): 170–172. https://doi.org/10.1080/02724936.1988.11748563.
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