Therapeutic Efficacy of Allyl isothiocyanate Evaluated on N-nitrosodiethylamine/Phenobarbital induced Hepatocarcinogenesis in Wistar Rats
Keywords:
Hepatocarcinogenesis, Allyl isothiocyanate, Antioxidants, Marker enzymes, α-fetoproteinAbstract
N-nitrosodiethylamine (NDEA) is a potential carcinogenic agent that induces liver cancer. To evaluate the chemotherapeutic effect of allyl isothiocyanate in the experimental model, Wistar male rats were administered a single dose of intraperitoneal (IP) injection of NDEA. Two weeks after administration of NDEA, Phenobarbital at the concentration of 0.05% was incorporated in rat chow for up to 14 successive weeks to promote liver cancer. Allyl isothiocyanate (AITC) (2mg/kg body weight) in addition to 0.5ml of corn oil was given orally daily. At the end of this experimental period, the rats were sacrificed and the blood samples were taken for biochemical studies. The levels of the marker enzymes for liver function were measured in serum. The results of the biochemical studies showed that NDEA administration followed by phenobarbital induces macro and microscopic liver tumors that increase the levels of marker enzymes and decreases the level of antioxidant in the serum in addition to the loss of body weight. Conclusively, the administration of AITC as a therapeutic treatment for hepatocarcinoma has significantly reduced tumor development and counteracted all the biochemical effects induced by NDEA.
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