Effective Biosurfactants production by Pseudomonas aeruginosa and its efficacy on different Oils


  • K. V. Sekar Department of Microbiology, The Oxford College of Science, Bangalore-102, India.
  • Sarita Kumari Department of Microbiology, The Oxford College of Science, Bangalore-102, India.
  • A. Nagasathya P.G. and Research Department of Microbiology, J.J. College of Arts and Science, Pudukkottai- 622404, India.
  • S. Palanivel P.G. and Research Department of Microbiology, J.J. College of Arts and Science, Pudukkottai- 622404, India.
  • Subramanyam Nambaru CAS in Marine Biology, Annamalai University, Parangipettai, Tamil Nadu, India.


Biosurfactant production, Pseudomonas aeruginosa, Test efficacy, Rhamnolipid


A rhamnolipid producing bacterium, Pseudomonas aeruginosa was isolated from contaminated soil with oily wastes. The Pseudomonas aeruginosa has grown with glucose and corn oil as a carbon source produced biosurfactant. This biosurfactant was purified by procedures that included chloroform-ethanol extraction and 0.05M bicarbonate treatments. The active compound was identified as rhamnolipid by using thin layer chromatography. The biosurfactant efficacy was tested on coconut oil, groundnut oil, sunflower oil and olive oil. The coconut oil responded better and gave a maximum level of 1cm than the olive oil, groundnut oil and sunflower oil.


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How to Cite

Sekar, K. V., Kumari, S., Nagasathya, A., Palanivel, S., & Nambaru, S. (2010). Effective Biosurfactants production by Pseudomonas aeruginosa and its efficacy on different Oils. Advances in BioScience, 1(1), 31–34. Retrieved from https://journals.sospublication.co.in/ab/article/view/9