Characterization of Chromobacterium violaceum Isolated from Spoiled Vegetables and Antibiogram of Violacein

Authors

  • G. Rajalakshmi Department of Biotechnology, Hindusthan College of Arts and Science, Coimbatore, India.
  • A. Sankaravadivoo Department of Botany, Government Arts College, Coimbatore, India.
  • S. Prabhakaran Department of Biotechnology, Hindusthan College of Arts and Science, Coimbatore, India.

Keywords:

Chromobacterium violaceum, Purple Colony, Violacein, Antibiotic Susceptibility

Abstract

Chromobacterium violaceum, a gram-negative, facultative anaerobic, non-sporing coccobacillus, producing a natural antibiotic called violacein was extracted from Chromobacterium violaceum MTCC 2656, collection of Chandigarh. The present study reports different types of microbial population along with Chromobacterium violaceum in 36 samples of spoiled vegetables. In the numerous microbial populations, three different strains (HMCCC 45, HMCCC 46 and HMCCC 47) were isolated and subjected to various morphological and biochemical tests. The results reveal that the three strains isolated, HMCCC 46 was similar to the standard except that HMCCC 46 colonies were dark violet in colour. The antibiotic sensitivity test of HMCCC 46 revealed that it was resistant to vancomycin, ampicillin, linezolid, and susceptible to colistin, oxacillin, gentamicin, norfloxacin, chloramphenicol, amikacin.

Downloads

Download data is not yet available.

References

Baron, E.J., Peterson, L.R. & Finegold, S.M. (1994). Bailey & Scott's diagnostic microbiology. 9th ed., Mosby, St. Louis. pp. 566-567.

Boisbaudran, L. (1882). Matière colorante se formant dans la colle de farine. Comp. Rend. Acad. Sci., 94: 562-563.

Bromberg, N. & Durán, N. (2001). Violacein transformation by peroxidases and oxidases: implications on its biological properties. J. Mol. Catal. B: Enzym., 11(4): 463–467. https://doi.org/10.1016/S1381-1177(00)00171-5.

Caldas, L.R., Leitão, A.A.C., Santos, S.M. & Tyrrell, R.M. (1978). Preliminary experiments on the photobiological properties of violacein. In: Tyrrell, R.M. (ed.), Proceedings of the International Symposium on Current Topics in Radiology and Photobiology, Rio de Janeiro: Academia Brasileira de Ciências. pp. 121–126.

Cappuccino, J.G. & Sherman, N. (2002). Microbiology: A Laboratory Manual. 6th Ed., Pearson Education Inc., San Francisco. pp. 491.

Harrigan, W.F. (1998). Laboratory Methods in Food Microbiology, 3rd Ed., Academic Press, London.

Hassan, H., Suntharalingam, S. & Dhillon, K.S. (1993). Fatal Chromobacterium violaceum septicaemia. Singapore Med. J., 34(5): 456–458.

Hungria, M., Chueire, L.M.O., Coca, R.G. & Megı́as, M. (2001). Preliminary characterization of fast growing rhizobial strains isolated from soyabean nodules in Brazil. Soil Biol. Biochem., 33(10): 1349–1361. https://doi.org/10.1016/S0038-0717(01)00040-2.

Kaufman, S.C., Ceraso, D. & Schugurensky, A. (1986). First case report from Argentina of fatal septicemia caused by Chromobacterium violaceum. J. Clin. Microbiol., 23(5): 956–958. https://doi.org/10.1128/jcm.23.5.956-958.1986.

Koneman, E.W., Allen, S.D., Janda, W.M., Schreckenberger, P.C. & Winn Jr, W.C. (1997). Color atlas and textbook of diagnostic microbiology. 5th edn., Lippincott-Raven Publishers, Philadelphia. pp. 1488.

Myers, J., Ragasa, D.A. & Eisele, C. (1982). Chromobacterium violaceum septicemia in New Jersey. J. Med. Soc. NJ, 79(3): 213–214.

NCCLS (2000). Performance standards for antimicrobial disk susceptibility tests. Approved standard, 7th ed., NCCLS document M2-A7, National Committee for Clinical Laboratory Standards, Wayne, PA.

Rettori, D. & Durán, N. (1998). Production, extraction and purification of violacein: an antibiotic pigment produced by Chromobacterium violaceum. World J. Microbiol. Biotechnol., 14(5): 685–688. https://doi.org/10.1023/A:1008809504504.

Richard, C. (1993). Chromobacterium violaceum, opportunist pathogenic bacteria in tropical and subtropical regions. Bull. Soc. Pathol. Exot., 86(3): 169–173.

Sorensen, R.U., Jacobs, M.R. & Shurin, S.B. (1985). Chromobacterium violaceum adenitis acquired in the northern United States as a complication of chronic granulomatous disease. Pediatr. Infect. Dis., 4(6): 701–702. https://doi.org/10.1097/00006454-198511000-00028.

Downloads

Abstract views: 34 / PDF downloads: 8

Published

2011-01-29

How to Cite

Rajalakshmi, G., Sankaravadivoo, A., & Prabhakaran, S. (2011). Characterization of Chromobacterium violaceum Isolated from Spoiled Vegetables and Antibiogram of Violacein. Advances in BioScience, 2(1), 18–22. Retrieved from https://journals.sospublication.co.in/ab/article/view/28

Issue

Section

Articles