Effects of Different Times of Cutting Soaking and Concentrations of IAA on Morphological features of Robinia pseudoacacia Stem Cuttings


  • Sargul A. Khudhur Department of Biology, Faculty of Science and Health, Koya University. Koya KOY45, Kurdistan Region – F.R. Iraq. http://orcid.org/0000-0001-9644-851X


IAA, Times, Vegetative propagation, Stem cuttings, Robinia pseudoacacia


This research was conducted in the open field of the Department of Biology, Faculty of Science and Health, Koya University, Kurdistan region, located at 36o N, 44o E and 570m of altitude above sea level. A factorial experiment with randomized complete block design (RCBD) with three replications was conducted to study the combined effect of different times of cutting soaking and concentrations of IAA. In this study four times of cutting soaking 0, 10, 30 and 60m (t0, t1, t2 and t3), four concentrations 0, 500, 800 and 1000 ppm (C0, C1, C2 and C3) and distilled water was used as control. When Robinia pseudoacacia was exposed to various concentrations of IAA and times of cutting soaking, it was found that IAA concentration and times of cutting soaking in R. pseudoacacia had significant effects on the shoot percentage, shoot height, number of leaves, number of main branches, diameter of main branches, leaf area, chlorophyll a, chlorophyll b, total chlorophyll, and carotenoid content. Showed significant effects such as an increase in some vegetative growth, leaves chemical content as compared with control. Overall, the results show that R. pseudoacacia stem cuttings are superior for a time of cutting soaking with ten minutes and IAA concentration with 500 ppm.


Download data is not yet available.


Alagesaboopathi, C. (2012). Influence of indole acetic acid and indole butyric acid on root development and status of Andrographis elongata (Vahl) T. and – an endemic medicinal plant of India. Int. J. Biosci., 2(4): 75-81.

Al-Barzinji, I.M., Khudhur, S.A. & Abdulrahman, Y.A. (2016). Non-Destructive Method for Prediction Leaf Area and Chlorophyll Content for Some Horticultural Plants Cultivated in Erbil City. Journal of University of Duhok, 19(1): 339-347.

Al-Sahaf, F.H. (1989). Applied Plant Nutrition. Ministry of Higher Education and Scientific Research, University of Baghdad. Al-Hikma House, Iraq, pp 260. (In Arabic).

Cierjacks, A., Kowarik, I., Joshi, J., Hempel, S., Ristow, M., von der Lippe, M. & Weber, E. (2013). Biological Flora of the British Isles: Robinia pseudoacacia. J. Ecol., 101(6): 1623–1640. https://doi.org/10.1111/1365-2745.12162.

Dick, J.M., Leakey, R.R., McBeath, C., Harvey, F., Smith, R.I. & Woods, C. (2004). Influence of nutrient application rate on growth and rooting potential of the West African hardwood Triplochiton scleroxylon. Tree Physiol., 24(1): 35–44. https://doi.org/10.1093/treephys/24.1.35.

Gehlot, A., Gupta, R.K., Tripathi, A., Arya, I.D. & Arya, S. (2014). Vegetative propagation of Azadirachta indica: effect of auxin and rooting media on adventitious root induction in mini-cuttings. Adv. For. Sci., 1(1): 1–9.

Hassanein, A.M.A. (2013). Factors Influencing Plant Propagation Efficiency via Stem Cuttings. J. Hort. Sci. Ornamen. Plants, 5(3): 171-176.

Huntley, J.C. (1990). Robinia pseudoacacia L. Black Locust. In: Burns, R.M. & Honkala, B.H. (Tech. Coords). Silvics of North America, Vol. 2: Hardwoods. Agriculture Handbook 654. U.S. Department of Agriculture, Forest Service, Washington, DC. pp. 755-761.

Kenney, G., Sudi, J. & Blackman, G.E. (1969). The Uptake of Growth Substances: XIII. DIFFERENTIAL UPTAKE OF INDOL-3YL-ACETIC ACID THROUGH THE EPIDERMAL AND CUT SURFACES OF ETIOLATED STEM SEGMENTS. J. Exp. Bot., 20(4): 820–840. https://doi.org/10.1093/jxb/20.4.820.

Khudhur, S.A. & Omar, T.J. (2015). Effect of NAA and IAA on Stem Cuttings of Dalbergia Sissoo (Roxb). Journal of Biology and Life Science, 6(2): 208–220. https://doi.org/10.5296/jbls.v6i2.7445.

Li, H., Li, J., He, Y., Li, S., Liang, Z., Peng, C., Polle, A. & Luo, Z.-B. (2013). Changes in carbon, nutrients and stoichiometric relations under different soil depths, plant tissues and ages in black locust plantations. Acta Physiol. Plant., 35(10): 2951–2964. https://doi.org/10.1007/s11738-013-1326-6.

Mbagwu, F.N., Ogbonnaya, C.I., Umeoka, N. & Edoki, N. (2017). Effects of indole-3-acetic acid (IAA) on the Vegetative propagation and Phytochemical properties of Bushbuck (Gongronema latifolium Benth.). J. Food Process. Technol., 8(1): 1–3. https://doi.org/10.4172/2157-7110.1000649.

Orwa, C., Mutua, A., Kindt, R., Simons, A. & Jamnadass, R.H. (2009). Agroforestree database: a tree reference and selection guide version 4.0. World Agroforestry Centre, Kenya.

Park, S. & Han, K.-H. (2003). An auxin-repressed gene (RpARP) from black locust (Robinia pseudoacacia) is posttranscriptionally regulated and negatively associated with shoot elongation. Tree Physiol., 23(12): 815–823. https://doi.org/10.1093/treephys/23.12.815.

Pijut, P.M., Woeste, K.E. & Michler, C.H. (2010). Promotion of Adventitious Root Formation of Difficult-to-Root Hardwood Tree Species. In: Janick, J. (Ed.), Horticultural Reviews. Vol. 38, John Wiley & Sons, Inc. New York. https://doi.org/10.1002/9780470872376.ch6.

Pop, T.I., Pamfil, D. & Bellini, C. (2011). Auxin Control in the Formation of Adventitious Roots. Not. Bot. Horti Agrobot. Cluj Napoca, 39(1): 307–316. https://doi.org/10.15835/nbha3916101.

Rahbin, A., Aboutalebi, A. & Khankahdani, H.H. (2012). Study on the effect of cutting location on shoot and IBA on rooting of 'Night Jessamine' (Cestrum nocturnum) stem cuttings. Intl. Res. J. Appl. Basic Sci., 3(11): 2345–2348.

Rai, P. & Mishra, R.M. (2013). Effect of urban air pollution on epidermal traits of road side tree species, Pongamia pinnata (L.) Merr. IOSR J. Environ. Sci. Toxicol. Food Technol., 2(6): 4–7. https://doi.org/10.9790/2402-0260407.

Rédei, K., Keserű, Z., Csiha, I., Rásó, J. & Antal, B. (2013). Juvenile Growth and Morphological Traits of Micropropagated Black Locust (Robinia pseudoacacia L.) Clones under Arid Site Conditions. Acta Silv. Lignaria Hung., 9: 35–42. https://doi.org/10.2478/aslh-2013-0003

Hoshmand, A.R. (2006). Design of Experiments for Agriculture and the Natural Sciences. 2nd Edition. Chapman and Hall/CRC Press, Boca Raton, FL. pp. 452.

Rice, S.K., Westerman, B. & Federici, R. (2004). Impacts of the exotic, nitrogen-fixing black locust (Robinia pseudoacacia) on nitrogen-cycling in a pine–oak ecosystem. Plant Ecol., 174(1): 97–107. https://doi.org/10.1023/B:VEGE.0000046049.21900.5a.

Ssekabembe, C.K., Henderlong, P.R. & Larson, M. (1994). Soil moisture relations at the tree/crop interface in black locust alleys. Agrofor. Syst., 25(2): 135–140. https://doi.org/10.1007/BF00705673.

Sevik, H. & Guney, K. (2013). Effects of IAA, IBA, NAA, and GA3 on rooting and morphological features of Melissa officinalis L. stem cuttings. The Scientific World Journal, 2013, 909507. https://doi.org/10.1155/2013/909507.

Swamy, S.L. Puri, S. & Kanwar, K. (2002). Propagation of Robinia pseudoacacia Linn. and Grewia optiva Drummond from rooted stem cuttings. Agrofor. Syst., 55(3): 231–237. https://doi.org/10.1023/A:1020579516144.

Swearingen, J. (2009). WeedUS - Database of Plants Invading Natural Areas in the United States: Black Locust (Robinia pseudoacacia). Retrieved from https://www.invasiveplantatlas.org/subject.cfm?sub=3350.

Taiz, L. & Zeiger, E. (1998). Plant Physiology. 2nd Edition, Sinauer Associates Publishers, Sunderland, Massachusetts. pp. 792.

Thomas, S.C. & Winner, W.E. (2000). Leaf area index of an old-growth Douglas-fir forest estimated from direct structural measurements in the canopy. Can. J. For. Res., 30(12): 1922-1930. https://doi.org/10.1139/x00-121.

Xiu, Y., Iqbal, A., Zhu, C., Wu, G., Chang, Y., Li, N., Cao, Y., Zhang, W., Zeng, H., Chen, S. & Wang, H. (2016). Improvement and transcriptome analysis of root architecture by overexpression of Fraxinus pennsylvanica DREB2A transcription factor in Robinia pseudoacacia L. 'Idaho'. Plant Biotechnol. J., 14(6): 1456–1469. https://doi.org/10.1111/pbi.12509.

Wang, Z., Yang, X., Wang, X.M. & Gao, H.W. (2011). Growth and physiological response of tall oat grass to salinity stress. Afr. J. Biotechnol., 10(37): 7183–7190. https://doi.org/10.5897/AJB11.809.

Zhang, J., Liu, Y. & Wang H. (2007). Micropropagation of Black Locust (Robinia pseudoacacia L.). In: Jain, S.M. & Häggman, H. (eds), Protocols for Micropropagation of Woody Trees and Fruits. Springer, pp. 193–199. https://doi.org/10.1007/978-1-4020-6352-7_18.


Abstract views: 17 / PDF downloads: 10



How to Cite

Khudhur, S. A. (2019). Effects of Different Times of Cutting Soaking and Concentrations of IAA on Morphological features of Robinia pseudoacacia Stem Cuttings. Advances in BioScience, 10(4), 111–122. Retrieved from https://journals.sospublication.co.in/ab/article/view/273