Conventional and molecular differentiation between capsular types of Pasteurella multocida isolated from various animal hosts


  • Ayaat Teleb Department of Genetics, Faculty of Agriculture, Fayoum University, Fayoum 63514, Egypt.
  • Gamal Mohamedin Hassan Department of Genetics, Faculty of Agriculture, Fayoum University, Fayoum 63514, Egypt.
  • Ahmed Yaseein Department of Genetics, Faculty of Agriculture, Fayoum University, Fayoum 63514, Egypt.
  • Zaki El Fiky Department of Genetics, Faculty of Agriculture, Fayoum University, Fayoum 63514, Egypt.


Pasteurella multocida, Hyaluronidase test, Acriflavine test, Capsular typing, PCR, Multiplex PCR


In this study, ten strains of P. multocida isolated from chicken, cattle, buffalo and sheep had a clinical manifestation of pneumonia were identified by species-specific PCR (PM-PCR) and 460bp products were obtained. Capsular typing of P. multocida is useful for epidemiological evidence and has been assessed by conventional and genotyping assays. According to the results, only one out of the ten strains (10%) which isolated from chicken was detected as capsular type D based on acriflavine test and did not detect the capsular type A for all the ten strains (0%) by using hyaluronidase test. PCR was applied to identify the capsular types using specific primers for each type of P. multocida. The findings of this study showed that a uniform amplicon size was corresponding to 657bp, 851bp and 510bp indicating that it belongs to capsular type D, type F and type E, respectively. So, the ten strains were identified to a one strain isolated from chicken as a type D (10%), one strain of which isolated from cattle as a type F (10%) and eight strains of which isolated from sheep, cattle, buffalo and chicken as a type E (80%). Likewise, the data of multiplex PCR showed that capsular type D, one strain; capsular type F, one strain and capsular type E, eight strains. Thus, the multiplex PCR can be used as a simple, sensitive, rapid, reliable technique for capsular typing identification of P. multocida. We concluded that the P. multocida serogroup E is common in Egypt and has a wide host range.


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Carter, G.R. (1967). Pasteurellosis: Pasteurella multocida and Pasteurella hemolytica. Adv. Vet. Sci., 11: 321–379.

Boyce, J.D., Harper, M., Wilkie, I.W. & Adler, B. (2010). Pasteurella. In: Gyles, C.L., Prescott, J.F., Songer, G. & Thoen, C.O. (eds), Pathogenesis of bacterial infections in animals. 4th ed., Ames: Blackwell Publishing. pp. 325-346.

Rimler, R.B. & Rhoades, K.R. (1987). Serogroup F, a new capsule serogroup of Pasteurella multocida. J. Clin. Microbiol., 25(4): 615–618.

Harper, M., Boyce, J.D. & Adler, B. (2006). Pasteurella multocida pathogenesis: 125 years after Pasteur. FEMS Microbiol. Lett., 265(1): 1–10.

Carter, G.R. (1955). Studies on Pasteurella multocida. I. A hemagglutination test for the identification of serological types. Am. J. Vet. Res., 16(60): 481–484.

Carter, G.R. (1972). Improved hemagglutination test for identifying type A strains of Pasteurella multocida. Appl. Microbiol., 24(1): 162–163.

St Michael, F., Harper, M., Parnas, H., John, M., Stupak, J., Vinogradov, E., Adler, B., Boyce, J.D. & Cox, A.D. (2009). Structural and genetic basis for the serological differentiation of Pasteurella multocida Heddleston serotypes 2 and 5. J. Bacteriol., 191(22): 6950–6959.

Carter, G.R. & Subronto, P. (1973). Identification of type D strains of Pasteurella multocida with acriflavine. Am. J. Vet. Res., 34(2): 293–294.

Carter, G.R. & Rundell, S.W. (1975). Identification of type A strains of P multocida using staphylococcal hyaluronidase. Vet. Rec., 96(15): 343.

Brogden, K.A. & Packer, R.A. (1979). Comparison of Pasteurella multocida serotyping systems. Am. J. Vet. Res., 40(9): 1332–1335.

Mutters, R., Ihm, P., Pohl, S., Frederiksen, W. & Mannheim, W. (1985). Reclassification of the Genus Pasteurella Trevisan 1887 on the Basis of Deoxyribonucleic Acid Homology, with Proposals for the New Species Pasteurella dagmatis, Pasteurella canis, Pasteurella stomatis, Pasteurella anatis, and Pasteurella langaa. Int. J. Syst. Evol. Microbiol., 35(3), 309–322.

Blackall, P.J., Pahoff, J.L. & Bowles, R. (1997). Phenotypic characterisation of Pasteurella multocida isolates from Australian pigs. Vet. Microbiol., 57(4): 355–360.

Townsend, K.M., Frost, A.J., Lee, C.W., Papadimitriou, J.M. & Dawkins, H.J. (1998). Development of PCR assays for species- and type-specific identification of Pasteurella multocida isolates. J. Clin. Microbiol., 36(4): 1096–1100.

Townsend, K.M., Boyce, J.D., Chung, J.Y., Frost, A.J. & Adler, B. (2001). Genetic organization of Pasteurella multocida cap Loci and development of a multiplex capsular PCR typing system. J. Clin. Microbiol., 39(3): 924–929.

Chung, J.Y., Zhang, Y. & Adler, B. (1998). The capsule biosynthetic locus of Pasteurella multocida A:1. FEMS Microbiol. Lett., 166(2): 289–296.

Boyce, J.D. & Adler, B. (2000). The capsule is a virulence determinant in the pathogenesis of Pasteurella multocida M1404 (B:2). Infect. Immun., 68(6): 3463–3468.

Shayegh, J., Atashpaz, S., Salehi, T.Z. & Hejazi, M.S. (2010). Potential of pasteurella multocida isolated from healthy and diseased cattle and buffaloes in induction of diseases. Bull. Vet. Inst. Pulawy, 54(3): 299–304.

AL-Shemmari, I.G. & Al-Judi, A.M. (2014). Molecular identification by multiplex polymerase chain reaction of Pasteurella multocida in cattle and buffaloes in Baghdad. Iraqi J. Vet. Med., 38(1): 99–106.

Arumugam, N.D., Ajam, N., Blackall, P.J., Asiah, N.M., Ramlan, M., Maria, J., Yuslan, S. & Thong, K.L. (2011). Capsular serotyping of Pasteurella multocida from various animal hosts - a comparison of phenotypic and genotypic methods. Trop. Biomed., 28(1): 55–63.

Ragavendhar, K., Thangavelu, A., Kirubaharan, J.J., Ronald, B.S. & Chandran, N.D. (2015). Prevalence of virulence associated genes in Pasteurella multocida isolates from Tamil Nadu. Indian J. Anim. Sci., 85(12): 1289–1292.

Shirzad Aski, H. & Tabatabaei, M. (2016). Occurrence of virulence-associated genes in Pasteurella multocida isolates obtained from different hosts. Microb. Pathog., 96: 52–57.

Hassan, G.M., EL-Feky, Z.A., Eissa, E.A. & Ahmed, A.T. (2015). Determination of the relationships between Pasteurella multocida isolated from different farm animals and their host range contacts in egypt using biochemical and molecular techniques. Egypt. J. Genet. Cytol., 44(2): 221–233.

Tillett, D. & Neilan, B.A. (2000). Xanthogenate nucleic acid isolation from cultured and environmental cyanobacteria. J. Phycol., 36(1): 251–258.

Prabhakar, P., Thangavelu, A., Kirubaharan, J.J. & Chandran, N.D. (2012). Isolation and Characterisation of P. Multocida Isolates from Small Ruminants and Avian Origin. TamilNadu J. Veterinary & Animal Sciences, 8(3): 131-137.

Balakrishnan, G. & Roy, P. (2012). Isolation, identification and antibiogram of Pasteurella multocida isolates of avian origin. Tamilnadu J. Veterinary & Animal Sciences, 8(4): 199-202.

Fernandez, S., Galapero, J., Gomez, L., Perez, C.J., Ramos, A., Cid, D., Garcia, A. & Rey, J. (2018). Identification, capsular typing and virulence factors of Pasteurella multocida isolates from Merino lambs in Extremadura (Southwestern Spain). Vet. Med., 63(3): 117–124.

Król, J., Bania, J., Florek, M., Pliszczak-Król, A. & Staroniewicz, Z. (2011). Polymerase chain reaction-based identification of clinically relevant Pasteurellaceae isolated from cats and dogs in Poland. J. Vet. Diagn. Invest., 23(3): 532–537.

Rimler, R.B. (1994). Presumptive identification of Pasteurella multocida serogroups A, D and F by capsule depolymerisation with mucopolysaccharidases. Vet. Rec., 134(8): 191–192.

Shivachandra, S.B., Kumar, A.A., Gautam, R., Singh, V.P., Saxena, M.K. & Srivastava, S.K. (2006). Identification of avian strains of Pasteurella multocida in India by conventional and PCR assays. Vet. J., 172(3): 561–564.

Ewers, C., Lübke-Becker, A., Bethe, A., Kiebling, S., Filter, M. & Wieler, L.H. (2006). Virulence genotype of Pasteurella multocida strains isolated from different hosts with various disease status. Vet. Microbiol., 114: 304–317.

Chandrasekaran, S., Hizat, K., Saad, Z., Johara, M.Y. & Yeap, P.C. (1991). Evaluation of combined Pasteurella vaccines in control of sheep pneumonia. Br. Vet J., 147(5): 437–443.

Zamri-Saad, M., Effendy, W.M., Maswati, M.A., Salim, N. & Sheikh-Omar, A.R. (1996). The goat as a model for studies of pneumonic pasteurellosis caused by Pasteurella multocida. Br. Vet. J., 152(4): 453–458.

Shayegh, J., Atashpaz, S. & Hejazi, M.S. (2008). Virulence Genes Profile and Typing of Ovine Pasteurella multocida. Asian J. Anim. Vet. Adv., 3(4): 206–213.

Ferreira, T.S., Felizardo, M.R., Sena de Gobbi, D.D., Gomes, C.R., Nogueira Filsner, P.H., Moreno, M., Paixão, R., Pereira, J. & Micke Moreno, A. (2012). Virulence genes and antimicrobial resistance profiles of Pasteurella multocida strains isolated from rabbits in Brazil. The Scientific World Journal, 2012, 685028.

Verma, S., Sharma, M., Katoch, S., Verma, L., Kumar, S., Dogra, V., Chahota, R., Dhar, P. & Singh, G. (2013). Profiling of virulence associated genes of Pasteurella multocida isolated from cattle. Vet. Res. Commun., 37(1): 83–89.

Varga, Z., Volokhov, D.V., Stipkovits, L., Thuma, A., Sellyei, B. & Magyar, T. (2013). Characterization of Pasteurella multocida strains isolated from geese. Vet. Microbiol., 163(1-2): 149–156.

Wilkie, I.W., Harper, M., Boyce, J.D. & Adler, B. (2012). Pasteurella multocida: diseases and pathogenesis. Curr. Top. Microbiol. Immunol., 361: 1–22.

Harper, M., Boyce, J.D. & Adler, B. (2012). The key surface components of Pasteurella multocida: capsule and lipopolysaccharide. Curr. Top. Microbiol. Immunol., 361: 39–51.

Pruimboom, I.M., Rimler, R.B., Ackermann, M.R. & Brogden, K.A. (1996). Capsular hyaluronic acid-mediated adhesion of Pasteurella multocida to turkey air sac macrophages. Avian Dis., 40(4): 887–893.


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Teleb, A., Hassan, G. M., Yaseein, A., & Fiky, Z. E. (2019). Conventional and molecular differentiation between capsular types of Pasteurella multocida isolated from various animal hosts. Advances in BioScience, 10(1), 1–7. Retrieved from