Isolation and Identification of Pathogenic Bacteria that Cause External Ocular Infections in Sabha City Libya

Authors

  • Abdelkader Alsanousi G. Elzen Faculty of Science, Sabha University, P.O. Box 18758, Sabha-Libya.
  • Alsadig Mohammed Abdalla Department of Microbiology, Faculty of Medicine, Sabha University, P.O. Box 18758, Sabha-Libya.

Keywords:

Ocular Infections, Isolation, Identification of pathogenic bacteria

Abstract

The aim of this study was the isolation and identification of the specific bacterial pathogens causing ocular infections in patients attending Sabha Eye Clinics. A total of 120 samples were collected using a sterile cotton swab from patients with conjunctivitis (aged between 1 to more than 60 years) attending different Eye Clinics in Sabha city (under the supervision of ophthalmologists). All samples were examined by Gram stain, culture on blood agar, mannitol salt agar and MacConkey agar. Different biochemical tests were studied. Results revealed that the species of bacteria isolated were: Staphylococcus aureus (40%), Coagulase-negative Staphylococci (20%), Klebsiella pneumoniae species (10%), Pseudomonas aeruginosa (22%) and E. coli 8%.

Downloads

Download data is not yet available.

References

Alvarez, H. & Tabbara, K.F. (1996). Infections of the eyelids, In: Tabbara, K.F. & Hyndiuk, R.A. (ed.), Infections of the eye, 2nd edn., Little Brown & Co., Boston, pp. 559-570.

Barrow, G.I. & Feltham, R.K.A. (2003). Cowan and Steel's Manual for the Identification of Medical Bacteria. 3rd edn., Cambridge University Press, Cambridge, pp. 45-120.

Bauman, R.W., Machunis-Masuoka, E. & Montgomery, J.E. (2012). Microbiology: With diseases by body system. 3rd edn., Boston: Benjamin Cummings.

Brinser, J.H. (1986). Ocular Bacteriology. In: Tabbara, K.F. & Hyndiuk, R.A. (eds.), Infections of the eye. 1st edn. Little Brown & Company, Boston. p. 115-50.

Esenwah, E. (2005). Isolation and identification of the microorganisms most prevalent in external eye infections as seen in an eye clinic in Owerri. Journal of the Nigerian Optometric Association, 12: 6-9. https://doi.org/10.4314/jnoa.v12i1.64449.

Hemavathi, Sarmah, P. & Shenoy, P. (2014). Profile of microbial isolates in ophthalmic infections and antibiotic susceptibility of the bacterial isolates: a study in an eye care hospital, bangalore. J. Clin. Diagn. Res., 8(1): 23–25. https://doi.org/10.7860/JCDR/2014/6852.3910.

McClellan, K.A. (1997). Mucosal defense of the outer eye. Surv. Ophthalmol., 42(3): 233–246. https://doi.org/10.1016/s0039-6257(97)00090-8.

Ogbolu, D.O., Alli, O., Ephraim, I.E., Olabiyi, F.A. & Daini, O.A. (2011). In-Vitro efficacy of antimicrobial agents used in the treatment of bacterial eye infections in Ibadan, Nigeria. African J. Clin. Exp. Microbiol., 12(3): 124-128. https://doi.org/10.4314/ajcem.v12i3.7.

Ryan, K.J., Ray, C.G. & Sherris, J.C. (2004). Sherris Medical Microbiology: An Introduction to Infectious Diseases. 4th Edn., Medical Publishing Division, McGraw-Hill Companies. http://dx.doi.org/10.1036/0838585299.

Sherwal, B.L. & Verma, A.K. (2008). Epidemiology of Ocular Infection due to Bacteria and Fungus – A Prospective Study. J. Med. Educ. Res., 10(3): 127-131.

Sharma, S. (1988). Ocular Microbiology. 1st edn., Aravind Eye Hospital and Postgraduate Institute of Ophthalmology, Madurai.

Stenson, S., Newman, R. & Fedukowicz, H. (1982). Laboratory studies in acute conjunctivitis. Arch. Ophthalmol., 100(8): 1275–1277. https://doi.org/10.1001/archopht.1982.01030040253009.

Tesfaye, T., Beyene, G., Gelaw, Y., Bekele, S. & Saravanan, M. (2013). Bacterial Profile and Antimicrobial Susceptibility Pattern of External Ocular Infections in Jimma University Specialized Hospital, Southwest Ethiopia. Am. J. Infect. Dis. Microbiol., 1(1): 13–20. https://dx.doi.org/10.12691/ajidm-1-1-3.

Downloads

Abstract views: 20 / PDF downloads: 6

Published

2015-10-01

How to Cite

Elzen, A. A. G., & Abdalla, A. M. (2015). Isolation and Identification of Pathogenic Bacteria that Cause External Ocular Infections in Sabha City Libya. Advances in BioScience, 6(4), 95–96. Retrieved from https://journals.sospublication.co.in/ab/article/view/196

Issue

Section

Articles

Most read articles by the same author(s)