Mutagenic Effects of Sodium Azide on the Quality of Maize Seeds

Authors

  • J. J. Eze Department of Biological Sciences, Ahmadu Bello University, Zaria, Nigeria.
  • A. Dambo Department of Biological Sciences, Ahmadu Bello University, Zaria, Nigeria.

Keywords:

Mutation, Mutagen, Mutagenic, Sodium Azide, Mutants, Maize, Varieties, Nutrients

Abstract

This project was conducted to determine the mutagenic effects of sodium azide on the quality of maize seed. Maize seeds were treated at six different concentrations of sodium azide namely; 0.00mM, 0.01mM, 0.02mM, 0.03mM, 0.04mM and 0.05mM. The seeds that were treated were of two varieties namely; Sammaz 18 and Sammaz 20. The result obtained showed that sodium azide was effective in causing mutagenic change in the quality of maize seeds in terms of growth rate and seed size. Significant differences (P<0.05) were observed in all the two varieties with respect to some of the traits studied and nutritional compositions studied. The number of days to 50% flowering and Nitrogen-free extract (%) increased significantly with an increase in concentrations of sodium azide. Chlorophyll-deficient mutants were observed in treatments 0.02mM, 0.03mM and 0.04mM which were striata and light green in colour. Dwarfed mutant was also recorded in treatment 0.04mM of Sammaz 20 maize variety. The project was carried out to find out the effectiveness of sodium azide on the mutagenesis of maize seeds, seed weight of mutant maize plant, nutrient content of the mutant maize plant and the morphological features of the mutant maize plant. I recommend that chemical mutagens like sodium azide to produce improved seed varieties like of maize plants that will meet the present global and national food need.

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References

Acharya, S.N., Thomas, J.E. & Basu, S.K. (2007). Improvement in the medicinal and nutritional properties of fenugreek (Trigonella foenum-graecum L.). In: Acharya, S.N. & Thomas, J.E. (eds), Advances in medicinal plant research, Research Signpost, Trivandrum, Kerala, India.

Adamu, A.K. & Aliyu, H. (2007). Morphogical effects of sodium azide on tomato (Lycopersicon esculentum Mill). Sci. World J., 2(4): 9-12. https://doi.org/10.4314/swj.v2i4.51755.

Adamu, A.K. (2004). Gamma rays (60Co) and thermal neutron induced mutants in popcorn (Zea mays var. praccox Sturt). Nigerian J. Sci. Res., 4(2): 52-63.

Adamu, A.K., Olorunju, P.E., Bate, J.A. & Ogunlade, O.T.E. (2002). Radiosensitivity and effective dose determination in Groundnut (Arachis hypogaea L.) irradiated with Gamma Rays (Cobalt 60) sources. J. Agr. Env., 3(1): 71-84.

Ahloowalia, B.S. & Maluszynski, M. (2001). Induced mutations – A new paradigm in plant breeding. Euphytica, 118(2): 167–173. https://doi.org/10.1023/A:1004162323428.

Bretagne-Sagnard, B., Fouilloux, G. & Chupeau, Y. (1996). Induced albina mutations as a tool for genetic analysis and cell biology in flax (Linum usitatissimum). J. Exp. Bot., 47(2): 189–194. https://doi.org/10.1093/jxb/47.2.189.

Deepalakshmi, A.J. & Kumar, C.R.A. (2004). Creation of genetic variability for different polygenic traits in blackgram (Vigna mungo (L.) Hepper) through induced mutagenesis. Legume Res., 27(3): 188-192.

Dubinin, N.P. (1961). Problems of radiation genetics. Oliver and Boyd, London.

Osorio, J., Fernández-Martínez, J., Mancha, M. & Garcés, R. (1995). Mutant Sunflowers with High Concentration of Saturated Fatty Acids in the Oil. Crop Sci., 35(3): 739–742. https://doi.org/10.2135/cropsci1995.0011183X003500030016x.

Gichner, T. & Velemínský, J. (1977). The very low mutagenic activity of sodium azide in Arabidopsis thaliana. Biol. Plant., 19(2): 153–155. https://doi.org/10.1007/BF02926758.

Girija, M. (2008). Studies on induced mutagenesis in cowpea (Vigna unguiculata (L.) Walp). M. Phil. Thesis. Annamalai University, Annamalainagar, Tamil Nadu.

Gnanamurthy, S., Dhanavel, D., Girija, M., Pavadai, P. & Bharathi, T. (2012). Effect of Chemical mutagenesis on Quantitative traits of Maize (Zea mays (L.). International Journal of Research in Botany, 2(4): 34-36.

Hagberg, A. (1962). Production of duplications in barley breeding. Hereditas, 48(1-2): 243–246. https://doi.org/10.1111/j.1601-5223.1962.tb01810.x.

Jones, J.A., Starkey, J.R. & Kleinhofs, A. (1980). Toxicity and mutagenicity of sodium azide in mammalian cell cultures. Mutat. Res., 77(3): 293–299. https://doi.org/10.1016/0165-1218(80)90064-6.

Ramani, G.M. & Jadon, B.S. (1991). Induced variability in groundnut in M2 Generation. Gujarat Agricultural University Research Journal, 16(2): 23-26.

Kamra, O.P. & Gollapudi, B. (1979). Mutagenic effects of sodium azide in Drosophila melanogaster. Mutat. Res., 66(4): 381–384. https://doi.org/10.1016/0165-1218(79)90049-1.

Lee, Y.I., Lee, I.S. & Lim, Y.P. (2002). Variations in Sweetpotato Regenerates from Gamma-ray Irradiated Embryogenic Callus. J. Plant Biotechnol., 4(4): 163–170.

Maluszynski, M., Nichterlein, K., Zanten, L. & Ahloowalia, B.S. (2000). Officially Released Mutant Varieties - The FAO/IAEA Database. INIS-XA—291, International Atomic Energy Agency (IAEA), Vienna, Austria, 84 pp.

Gramatikova, M. & Todorov, I. (1996). Mutagenic specificity recording micro and macro mutants induction. Barley Genet. Newsl., 27: 68-72.

Owais, W.M. & Sadiq, M.F. (1993). O-acetyl-serine sulfhydrylases from different organisms converts azide to a mutagenic metabolite: Ability of the metabolite to inhibit the enzyme in vitro. Abhath Al-Yarmouk, 2(2): 43-55.

Pavadai, P. & Dhanavel, D. (2004). Effect of EMS, DES and colchicine treatment in soybean. Crop Research, 28: 118-120.

Pugalendi, N. (1992). Investigation on induced mutagenesis in Sesamum indicum L. M.Sc. (Ag.) Thesis, Annamalai University, Annamalai Nagar, Tamil Nadu.

Ramaswamy, N.M. (1973). Investigation on induced mutagenesis in black gram (Phaseolus mungo L.). Ph.D. Thesis, Tamil Nadu Agri. Univ., Coimbatore.

Rao, G.M. & Rao, V.M. (1983). Mutagenic Efficiency, Effectiveness and Factor of Effectiveness of Physical and Chemical Mutagens in Rice. Cytologia, 48(3): 427–436. https://doi.org/10.1508/cytologia.48.427.

Montalván, R. & Ando, A. (1998). Effect of gamma-radiation and sodium azide on quantitative characters in rice (Oryza sativa L.). Genet. Mol. Biol., 21: 81–85. https://doi.org/10.1590/S1415-47571998000100014.

Rines, H.W. (1985). Sodium azide mutagenesis in diploid and hexaploid oats and comparison with ethyl methanesulfonate treatments. Environ. Exp. Bot., 25(1): 7–16. https://doi.org/10.1016/0098-8472(85)90043-7.

Rizwana, B.M., Kalamani, A., Ashok, S. & Makesh, S. (2005). Effect of mutagenic treatments on qualitative characters in M1 generation of cowpea (Vigna unguiculata (L.) Walp). Adv. Plant Sci., 18(2): 505-510.

Sasi, A., Dhanavel, D. & Pavadai, P. (2005). Effect of chemical mutagenesis on bhendi (Abelmoschus esculentus (L.) Moench.) var. MDU 1. Research on Crops, 6(2): 253-255.

Singh, S.D., King, S.B. & Reddy, P.M. (1990). Registration of Five Pearl Millet Germplasm Sources with Stable Resistance to Downy Mildew. Crop Sci., 30(5): 1164. https://doi.org/10.2135/cropsci1990.0011183X003000050060x.

Tarar, J.L. & Dnyansagar, V.R. (1980). Comparison of ethyl methane sulphonate and radiation induced meiotic abnormalities in Turnera ulmifolia Linn. var. angustifolia Wild. Cytologia, 45: 221-231.

van den Bulk, R.W., Löffler, H.J., Lindhout, W.H. & Koornneef, M. (1990). Somaclonal variation in tomato: effect of explant source and a comparison with chemical mutagenesis. Theor. Appl. Genet., 80(6): 817–825. https://doi.org/10.1007/BF00224199.

Wen, J.Q. & Liang, H.G. (1995). Effect of KCN and NaN3 pretreatment on the cyanide resistant respiration in tobacco callus. Acta Bot. Sin., 37: 711-717.

Yuan, H.Y & Zhang, Z.L. (1993). Effect of Free Radicals and Temperature on Sister Chromatid Exchanges in Hordeum vulgare L. Acta Bot. Sin., 35: 20-26.

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Published

2015-07-01

How to Cite

Eze, J. J., & Dambo, A. (2015). Mutagenic Effects of Sodium Azide on the Quality of Maize Seeds. Advances in BioScience, 6(3), 76–82. Retrieved from https://journals.sospublication.co.in/ab/article/view/187

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