Evaluation of Genetic diversity and heritability of some rapeseed (Brassica napus) genotypes using agronomical and molecular traits
Keywords:
Brassica napus, Heritability, Genetic diversity, RAPD-PCRAbstract
High level of heritability and variability were estimated for all traits among genotypes. The genetic similarity of nine genotypes Brassica napus was estimated by random amplified polymorphic DNA (RAPD), which ranged from 69% to 94%. The phylogenetic tree resulted in two different clusters; the first including two genotypes which also showed high similarity in agronomic traits and the second included the rest of the genotypes. The genetic diversity was discussed in relation to molecular and agronomical traits. High and positive correlation were recorded for all traits. The high coupled of heritability and variability is considered as a powerful source for the selection of donors in the breeding program, where the genetic gain depends on the availability of genetic variation and high heritability.
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References
Prakash, S. & Hinata, K. (1980). Taxonomy, cytogenetics and origin of crop Brassicas, a review. Stackholm: Opera Botanica 55, 57 p.
Shukla, S., Bhargava, A., Chatterjee, A., Srivastava, A. & Singh, S.P. (2006). Genotypic variability in vegetable amaranth (Amaranthus tricolor L.) for foliage yield and its contributing traits over successive cuttings and years. Euphytica, 151(1): 103–110. https://doi.org/10.1007/s10681-006-9134-3.
Chao-zhi, M.A., Ting-dong, F.U., Tuevesson, S. & Gertsson, B. (2003). Genetic diversity of Chinese and Swedish rapeseed (Brassica napus L.) analysed by inter-simple sequence repeats (ISSRs). Plant Genet. Resour., 1(2-3): 169–174. https://doi.org/10.1079/PGR200326.
Kiran Patro, T.S. & Ravisankar, C. (2004). Genetic variability and multivariate analysis in okra (Abelmoschus esculentus (L.) Moench). Trop. Agric. Res., 16: 99-113.
Riaz, A., Li, G., Quresh, Z., Swati, M.S. & Quiros, C.F. (2001). Genetic diversity of oilseed Brassica napus inbred lines based on sequence-related amplified polymorphism and its relation to hybrid performance. Plant Breed., 120(5): 411–415. https://doi.org/10.1046/j.1439-0523.2001.00636.x.
Yuan, M., Zhou, Y. & Liu, D. (2004). Genetic diversity among populations and breeding lines from recurrent selection in Brassica napus as revealed by RAPD markers. Plant Breed., 123(1): 9–12. https://doi.org/10.1046/j.0179-9541.2003.00903.x.
Saha, S., Molla, R., Chandra, D. & Rahman, L. (2008). Assessment of genetic variation and relationships within the varieties of four Brassica species by RAPD markers. Aust. J. Crop Sci., 2(3): 105-114.
Ana, M.J., Ankica, K.S., Dejana, S.P., Radovan, M. & Nikola, H. (2009). Phenotypic and molecular evaluation of genetic diversity of rapeseed (Brassica napus L.) genotypes. Afr. J. Biotechnol., 8(19): 4835-4844.
Moghaddam, M., Mohammmadi, S.A., Mohebalipour, N., Toorchi, M., Aharizad, S. & Javidfar, F. (2009). Assessment of genetic diversity in rapeseed cultivars as revealed by RAPD and microsatellite markers. Afr. J. Biotechnol., 8(14): 3160-3167.
Asghari, A., Shokrpour, M., Chamanabad, H.M. & Sofalian, O. (2011). Evaluating genetic diversity of canola cultivars using morphological traits and molecular markers. Rom. Biotechnol. Lett., 16(4): 6305-6312.
Fahmi, A.I., Assal, O.M., Nawar, A.A., El-Hosary, A.A. & Mohammed, M.M. (2012). Genetic Diversity of Brassica napus L. Varieties Estimated by Morphological and Molecular Markers. Int. J. Plant Breed. Genet., 6: 83-93. https://dx.doi.org/10.3923/ijpbg.2012.83.93.
Qu, C., Hasan, M., Lu, K., Liu, L., Liu, X., Xie, J., Wang, M., Lu, J., Odat, N., Wang, R., Chen, L., Tang, Z. & Li, J. (2012). Genetic diversity and relationship analysis of the Brassica napus germplasm using simple sequence repeat (SSR) markers. Afr. J. Biotechnol., 11(27): 6923–6933. https://doi.org/10.5897/AJB11.3475.
Özbek, Ö. & Gidik, B.U. (2013). Genetic Diversity in Commercial Rapeseed (Brassica napus L.) varieties from Turkey as revealed by RAPD. Not. Sci. Biol., 5(1): 114–119. https://doi.org/10.15835/nsb518911.
Ausubel, F.M., Brent, R., Kingston, R.E., Moore, D.D., Seidman, J.G., Smith, J.A. & Struhl, K. (eds) (1987). Current Protocols in Molecular Biology. John Wiley & Sons, New York.
Konzak, C.F., Wickham, I.M. & DeKock, M.J. (1972). Advances in methods of mutagen treatment. In: Induced Mutations and Plant Improvement, STI/PUB/297, International Atomic Energy Agency, Vienna, pp. 95–119.
Marjanović-Jeromela, A., Nagl, N., Gvozdanović-Varga, J., Hristov, N., Kondić-Špika, A. & Marinković, M.V.R. (2011). Genotype by environment interaction for seed yield per plant in rapeseed using AMMI model. Pesqui. Agropecu. Bras., 46: 174–181. https://doi.org/10.1590/S0100-204X2011000200009.
Chand, N., Vishwakarma, S.R., Verma, O.P. & Manoj, K. (2008). Worth of genetic parameters to sort out new elite barley lines over heterogeneous environments. Barley Genet. Newslett., 8: 10-13.
Singh, A. (2011). Genetic variability in two-rowed barley (Hordeum vulgare L.). Indian J. Sci. Res., 2(3): 21-23.
Seyis, F., Snowdon, R.J., Luhs, W. & Friedt, W. (2003). Molecular characterization of novel resynthesized rapeseed (Brassica napus) lines and analysis of their genetic diversity in comparison with spring rapeseed cultivars. Plant Breed., 122(6): 473–478. https://doi.org/10.1111/j.1439-0523.2003.00859.x.
Snowdon, R.J. & Friedt, W. (2004). Molecular markers in Brassica oilseed breeding: current status and future possibilities. Plant Breed., 123(1): 1–8. https://doi.org/10.1111/j.1439-0523.2003.00968.x.
Singh, S.P., Srivastava, A.N. & Katiyar, R.P. (1979). Path analysis in Indian colza. Indian J. Genet. Plant Breed., 39: 150-153.
Thakral, N.K. (1982). To study the association of some morpho-physiological attributes with seed yield in toria (Brassica campestris L. var. toria). M.Sc. Thesis Abst., Haryana Agric. Univ., Hisar, India. 8(1): 66-67.
Das, M.K., Rahman, L. & Quddus, M.A. (1984). Correlation and path analyses in parents and F3 of Brassica juncea X Brassica campestris. Bangladesh J. Agric., 9: 1-12.
Pathak, S.C., Chaudhry, F.P. & Dixit, S.K. (1986). Selection criteria for seed yield in mustard (B. Juncea L.). Madras Agric. J., 71: 758-762.
Khatri, A., Khan, I.A., Siddiqui, M.A., Raza, S. & Nizamani, G.S. (2005). Evaluation of high yielding mutants of Brassica juncea cv. S-9 developed through gamma rays and EMS. Pak. J. Bot., 37(2): 279-284.
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