Involvement of HLA in Serum Beta-2 Microglobulin levels among Mumbai HIV-1 Patients

Authors

  • U. Shankarkumar National Institute of Immunohaematology, 13th Floor, KEM Hospital, Parel, Mumbai - 400012, Maharashtra, India.
  • A. Shankarkumar National Institute of Immunohaematology, 13th Floor, KEM Hospital, Parel, Mumbai - 400012, Maharashtra, India.
  • K. Ghosh National Institute of Immunohaematology, 13th Floor, KEM Hospital, Parel, Mumbai - 400012, Maharashtra, India.

Keywords:

HIV patients, B2M, HLA, Mumbai

Abstract

HLA allele involves along with B-2 microglobulin (B2M) and T cell receptor in host specific immune response. HLA alleles are known for its extensive polymorphism in order to accommodate the maximum of antigenic peptides. Serum beta-2 microglobulin has been reported to have a prognostic value for predicting disease progression in HIV patients. In this study, we evaluated the serum B2M levels and the HLA profile among the 124 HIV patients and 94 Normal healthy controls from Mumbai. The B2M levels were estimated by conventional ELISA technique while the HLA A and HLA B were done using PCR – SSOP molecular kits. Our results revealed that HIV patients showed increased B2M levels (88.70%) range (2.5ug/ml – 60ug/ml) compared the controls (42.55%). HLA A*11:01:01 (OR=3.25; P value 0.0015), HLA B*35:01:01 OR=3.57;P value 0.0002), HLA B*44:03:01 (OR=5.32; P value 0.0036), haplotypes HLA A*11:01:01- B*35:01:01 (OR=5.68; P value 2.47E-05), HLA A*33:03:01- B* 44:03:01 (OR=19.95; P value 0.0003) were significantly increased among the B2M increased HIV patients. While HLA A*03:01:01:01(OR=0.18; P value 0.0061) was significantly decreased among B2M increased HIV patients. Our results reveal that the production of serum B2M levels correlates with the HIV disease progression. Therefore it could be of a prognostic value in HIV infection.

Downloads

Download data is not yet available.

References

Sonkar, G.K., Usha, & Singh, R.G. (2008). A preliminary study on the significant value of beta-2-microglobulin over serum creatinine in renal transplant rejection and renal failure. Singapore Med. J., 49(10): 786–789.

Miller, S.A., Dykes, D.D. & Polesky, H.F. (1988). A simple salting out procedure for extracting DNA from human nucleated cells. Nucleic Acids Res., 16(3): 1215. https://doi.org/10.1093/nar/16.3.1215.

Mack, S.J. & Hollenbach, J.A. (2010). Allele Name Translation Tool and Update NomenCLature: software tools for the automated translation of HLA allele names between successive nomenclatures. Tissue Antigens, 75(5): 457–461. https://doi.org/10.1111/j.1399-0039.2010.01477.x.

Cooper, E.H., Forbes, M.A. & Hambling, M.H. (1984). Serum beta 2-microglobulin and C reactive protein concentrations in viral infections. J. Clin. Pathol., 37(10): 1140–1143. https://doi.org/10.1136/jcp.37.10.1140.

Bäckman, L., Ringdén, O., Björkhem, I. & Lindbäck, B. (1986). Increased serum β2 microglobulin during rejection, cyclosporine-induced nephrotoxicity, and cytomegalovirus infection in renal transplant recipients. Transplantation, 42(4): 368–371. https://doi.org/10.1097/00007890-198610000-00007.

Corlin, D.B., Sen, J.W., Ladefoged, S., Lund, G.B., Nissen, M.H. & Heegaard, N.H. (2005). Quantification of cleaved β2-microglobulin in serum from patients undergoing chronic hemodialysis. Clin. Chem., 51(7): 1177–1184. https://doi.org/10.1373/clinchem.2005.049544.

Jovanović, D., Krstivojević, P., Obradović, I., Durdević, V. & Dukanović, L. (2003). Serum cystatin C and β2-microglobulin as markers of glomerular filtration rate. Ren. Fail., 25(1): 123–133. https://doi.org/10.1081/jdi-120017475.

Child, J.A. & Kushwaha, M.R. (1984). Serum beta 2-microglobulin in lymphoproliferative and myeloproliferative diseases. Hematol. Oncol., 2(4): 391–401. https://doi.org/10.1002/hon.2900020409.

Garden, G.A., Moss, G.B., Emonyi, W., Bwayo, J., Velentgas, P. & Kreiss, J. (1993). Beta-2 microglobulin as a marker of HIV disease status in Nairobi, Kenya. Int. J. STD AIDS, 4(1): 49–51. https://doi.org/10.1177/095646249300400110.

Shankarkumar, U., Thakar, M., Mehendale, S., Praranjape, R.S. & Mohanty, D. (2003). Association of HLA B*3520, B*1801, and Cw*1507 with HIV-1 infection Maharashtra, India. J. Acquir. Immune Defic. Syndr., 34(1): 113–114. https://doi.org/10.1097/00126334-200309010-00017.

Shankarkumar, U. (2004). HIV and human leukocyte antigen association--the Indian scenario. J. HIV Ther., 9(3): 60–64.

Shankarkumar, U., Pawar, A. & Ghosh, K. (2007). Specific human leukocyte antigen alleles associated with HIV-1 infection in an Indian population. J. Acquir. Immune Defic. Syndr., 44(4): 489–490. https://doi.org/10.1097/QAI.0b013e31803133d8.

Downloads

Abstract views: 28 / PDF downloads: 15

Published

2011-07-03

How to Cite

Shankarkumar, U., Shankarkumar, A., & Ghosh, K. (2011). Involvement of HLA in Serum Beta-2 Microglobulin levels among Mumbai HIV-1 Patients. Advances in BioScience, 2(3), 126–128. Retrieved from https://journals.sospublication.co.in/ab/article/view/42

Issue

Vol. 2 No. 3 (2011): July

Section

Articles

License

Copyright (c) 2011 The author(s) retains the copyright of this article.

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.