Molecular Markers in determining Phylogenetic relationship in Foxtail Millet (Setaria italica (L.) P. Beauv.) Var. Co(Te)7
Keywords:
Setaria italica, M2 generation, RAPD, Primers, MutantsAbstract
The degree of polymorphism in seven different mutants were assessed along with untreated population, control in Setaria italica var. Co(Te)7. The mutants identified in M2 generation were selected for RAPD analysis to examine the genetic variation between them. The mutant like multiple branched panicle, long Panicle, tall plant, anthocyanin pigmentation in panicle, thumb branched panicle, bold seed, tiller were chosen to compare the banding pattern and distinguishing to evaluate their phylogenetic relationship. The primers chosen for their amplification were OPC11, OPA05, OPA08 and OPA13. All the mutants showed the polymorphism depicting genetic diversity and phylogenetic relationship in the foxtail millet var. Co(Te)7. Due to the exclusive design of genetic background, therapeutic potential, nutritional benefits and the traits of agronomic values hike the need for cultivation methodology in Co(Te)7 variety in foxtail millet. The dendrogram constructed based on UPGMA clustering method revealed the diverse genotype between the essential mutants utilized for increased crop improvement program.
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